OMIPs Directory

OMIP-021

Simultaneous quantification of human conventional and innate-like T-cell subsets

Cytometry PART A, Volume 85, Issue 7, 573-575 (2014)
Nicholas A. Gherardin, David S. Ritchie, Dale I. Godfrey, Paul J. Neeson

Description: This panel was developed in order to simultaneously quantify both conventional peptide-MHC-restricted, and innate-like T-cell compartments in human peripheral blood samples. The panel can assess the dynamics of naïve through to terminally differentiated effector memory T-cell subsets, as well as enumerating natural killer T (NKT) cells, mucosal-associated invariant T (MAIT) cells, γδ T-cells, and subsets thereof. The panel is suitable for use on both, freshly isolated or cryopreserved peripheral blood mononuclear cells (PBMC). Staining may be performed in a 96-well plate to increase throughput.

Species: Human

Tissue: Peripheral blood

Cell Subset: T cell, T memory, Tcrgd, Mait, Ilc, Nk, Nkt

Keyword: Cryopreserved pbmc

Machine: BD LSR Fortessa

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
CCR7 Human 3D12 PE-Cy7 memory T cell subset TCRgd, T memory Low
CD45RA Human HI100 PerCP-Cy5.5 memory T cell subset TCRgd, T memory Med
Dead cells Human Live/Dead Fix Aqua Viability (dump)
CD4 Human RPA-T4 APC-Cy7 Phenotyping T memory High
CD8a Human RPA-T8 Brilliant Violet 650 Phenotyping TCRgd, NKT, MAIT High
CD8b Human 2ST8.5H7 APC Phenotyping T memory Med
CD27 Human O323 Brilliant Violet 711 memory T cell subset TCRgd, T memory Med
CD28 Human 28.2 PE-Cy5 memory T cell subset NKT, MAIT, TCRgd, T memory Med
CD45RO Human UCHL1 Alexa Fluor 700 memory T cell subset T memory, NKT, MAIT High
CD161 Human HP-3G10 Brilliant Violet 605 MAIT MAIT Low
TCR gamma/delta Human 11F2 FITC γδT cell TCRgd Low
TCR V alpha 7.2 Human 3C10 PE MAIT MAIT Med
CD3 Human OKT3 Brilliant Violet 785 Lineage TCRgd, T memory High
CD1d/PBS44 Human Brilliant Violet 421 NKT NKT