OMIP-020
Phenotypic characterization of human γδ T-cells by multicolor flow cytometry
Cytometry PART A, Volume 85, Issue 6, 522-524 (2013)
Kilian Wistuba-Hamprecht, Graham Pawelec, Evelyna Derhovanessian
Description: This panel was composed and optimized to investigate the differentiation stages of human γδ T-cells in cryopreserved peripheral blood mononuclear cells (PBMC) from healthy individuals. As the majority of pan-γδ T-cell antibodies available commercially proved to be inappropriate for detecting all γδ T-cell populations in combination with other markers, this panel provides an essential tool for the analysis of different subsets of human γδ T-cells by flow cytometry. The panel works very well with cryopreserved PBMC. Other tissues have not been tested.
Species: Human
Tissue: Peripheral blood
Cell Subset: T cell, T memory, Tcrgd, Tcr vδ1, Tcr vδ2
Keyword: Cryopreserved pbmc
Machine: BD LSR2
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| Dead cells | Human | EMA | viability | |||
| CD28 | Human | CD28.2 | PE | differentiation | TCRgd, T memory | Med |
| CD3 | Human | UCHT1 | Alexa Fluor 700 | Lineage | TCRgd | High |
| CD4 | Human | OKT4 | PE-Cy7 | CD4 T cell | High | |
| TCR gamma/delta | Human | 11F2 | Purified | γδT cell | TCRgd | Low |
| IgG | Human | N/A | Pacific Orange | secondary reagent to TCRγδ (clone qqF2) | TCRgd | |
| TCR V delta 1 | Human | TS8.2 | FITC | γδT cell subset | TCRgd | Med |
| TCR V delta 2 | Human | B6 | PerCP | γδT cell subset | TCRgd | Med |
| CD8a | Human | SK1 | APC-H7 | CD8 T cell | TCRgd | High |
| CD45RA | Human | HI100 | Pacific Blue | differentiation | TCRgd, T memory | Med |
| CD16 | Human | 3G8 | Brilliant Violet 711 | γδT activation | TCRgd, T memory | Med |
| CD27 | Human | O323 | APC | differentiation | TCRgd, T memory | Med |
