OMIPs Directory

OMIP-002

Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes

Chattopadhyay PK, Roederer M, Price DA. OMIP-002: Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes. Cytometry A. 2010;77(9):821-822. doi:10.1002/cyto.a.20945
Pratip K Chattopadhyay, Mario Roederer, David A. Price

Description: This panel was developed to determine the phenotype of human antigen (Ag)-specific CD8+ T-cells. Ag-specificities are identified by four peptide-major histocompatibility complex (MHC) class I (pMHCI) multimers (e.g., against Epstein-Barr virus (EBV) and cytomegalovirus (CMV) epitopes). Six markers of T-cell phenotype are used. This panel has been tested on fresh and cryopreserved peripheral blood mononuclear cells (PBMC), as well as bone marrow samples; staining may be performed in 96-well plates to increase throughput.

Species: Human

Tissue: Bone marrow, Peripheral blood

Cell Subset: T cell, T memory

Keyword: Antigen-specific t cell response, Cryopreserved pbmc, Cytomegalovirus (cmv), Epstein-barr virus (ebv), Tetramer

Machine: LSR II

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
CD14 Human exclusion High
CD127 Human R34.34 PE-Cy5.5 T cell memory subsets T memory Medium
Biotin Human APC secondary reagent to PD-1
CD45RO Human UCHL1 APC-Alexa 700 T cell memory subsets T memory High
CD4 Human MT-477 Qdot 705 T cell subset T memory High
PD-1 Human MIH4 T cell memory subsets T memory Low
Dead cells Human Live/Dead Fix Violet viability
CD57 Human NK-1 FITC T cell function T memory High
CD27 Human 1A4 PE-Cy5 T cell memory subsets T memory Medium
CCR7 Human 150503 APC-Alexa 750 T cell memory subsets T memory Low
CD19 Human HIB19 Pacific Blue exclusion Low
CD3 Human SK7 APC-Cy7 T cell subset T memory High