OMIPs Directory

OMIP-019

Quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors

Cytometry PART A, Volume 83A, Issue 8, 676-678 (2013)
Yolanda D. Mahnke, Margaret H. Beddall, Mario Roederer

Description: The present panel was optimized to quantify the relative frequencies of γδT-cells, invariant natural killer T-cells (iNKT-cells), and hematopoietic precursors in peripheral blood mononuclear cells (PBMC) from healthy individuals. It works well with cryopreserved PBMC and we have observed similar results with fresh specimens. Other tissue types have not been tested.

Species: Human

Tissue: Peripheral blood

Cell Subset: T cell, T memory, Tcrgd, Nk, Nkt

Keyword: Cryopreserved pbmc

Machine: BD LSR2

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
TCR V delta 1 Human TS8.2 FITC γδ T cell TCRgd Low
CCR7 Human 150503 Alexa Fluor 680 phenotyping T memory, T cell, TCRgd Low
CD27 Human 1A4LDG phenotyping T cell, TCRgd, T memory Med
CD1d/PBS-57 Human iNKT iNKT
CD3 Human OKT3 Brilliant Violet 785 lineage T cell, T memory, TCRgd, iNKT High
Dead cells Human Live/Dead Fix Aqua viability
CD28 Human CD28.2 PE-Cy5 Surface T cell, TCRgd, T memory Med
CD4 Human OKT4 Brilliant Violet 605 phenotyping T cell, T memory High
CD34 Human HI100 Brilliant Violet 421 Hematopoietic stem cell Med
TCR V gamma 9 Human B3 APC γδT cell TCRgd Med
CD45RA Human MEM-56 PE-Cy5.5 phenotyping T cell, TCRgd, T memory Med
TCR V delta 2 Human B6 Alexa Fluor 594 γδT cell TCRgd Med
CCR5 Human 2D7/CCR5 APC-Cy7 phenotyping T cell, T memory Med
CD8a Human RPA-T8 Qdot 585 phenotyping T cell, TCRgd High