OMIPs Directory

OMIP-018

Chemokine receptor expression on human T helper cells

Cytometry PART A, Volume 83A, Issue 6, 530-532 (2013)
Tess Brodie, Elena Brenna, Federica Sallusto

Description: This panel was developed for the enumeration of chemokine receptor expression on CD4 T cells from the naïve (Tn), stem cell memory (Tscm), central memory (Tcm), and effector memory (Tem) cell subsets. Eight chemokine receptors were chosen based upon previously published observations implicating their preferential expression on T helper type 1 (Th1), Th2, Th17, or Th22 cells, and their role in controlling lymphocyte migration to secondary lymphoid tissues, B cell follicles or non-lymphoid tissues, both in the steady state and during immune responses to pathogens, autoantigens, or allergens. This panel has been optimized for use with fresh peripheral blood mononuclear cells (PBMCs) with the observation that 24 h after blood draw, the expression of some chemokine receptors is reduced. Optimal staining requires PBMCs to be processed and acquired within 5 h of blood draw and may be performed in a 96-well plate format for high throughput experiments.

Species: Human

Tissue: Peripheral blood

Cell Subset: T cell, T helper, T memory, Th1, Th17, Th2, Th22, Ctl

Machine: BD LSR Fortessa

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
Human
CD185 Human 51505 Qdot 605 polarization T cell, T helper Med
CD95 Human Dx2 PerCP-eFluor 710 maturity T cell, T memory Low
CD194 Human 1G1 PE-Cy7 polarization T cell, T helper, Th2 Med
CD183 Human 1C6/CXCR3 PE-Cy5 polarization T cell, T helper Med
CCR5 Human 2D7/CCR5 APC-Cy7 polarization T cell Med
CD193 Human 61828 APC polarization T cell, Th2 Med
CD294 Human BM16 FITC polarization T cell, Th2 Med
CD45RA Human MEM-56 Qdot 655 maturity T cell, T memory Med
CCR10 Human 314305 PE polarization T cell, T helper Low
CD14 Human M5E2 V500 exclusion High
CD19 Human HIB19 V500 exclusion Low
Dead cells Human Live/Dead Fix Aqua viability/exclusion
CCR7 Human G043H7 Brilliant Violet 421 maturity T cell, T memory Low
CD3 Human 7D6 PE-Cy5.5 T cell T cell, T memory, T helper High
CD4 Human S3.5 PE-Texas Red T cell T cell, T memory, T helper High
CD16 Human 3G8 V500 exclusion Med
CCR6 Human 11A9 polarization T cell, T helper Med
Streptavidin Human Qdot 800 secondary reagent to CCR6 T cell, T helper