OMIP-015
Human regulatory and activated T-cells without intracellular staining
Cytometry PART A, Volume 83A, Issue 2, 179-181 (2013)
Yolanda D. Mahnke, Margaret H. Beddall, Mario Roederer
Description: The present panel was optimized to investigate the frequency and phenotype of regulatory T-cells (Treg), as well as the activation status of CD4+ and CD8+ T-cells in peripheral blood mononuclear cells (PBMC) from healthy individuals, without the use of intracellular staining (i.e., excluding the use of the canonical Treg marker, FoxP3). The panel has been developed using cryopreserved PBMC and we have observed similar results with fresh specimens. Other tissue types have not been tested.
Species: Human
Tissue: Peripheral blood
Cell Subset: T cell, T memory, Treg
Keyword: Cryopreserved pbmc
Machine: BD LSR2
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD4 | Human | OKT4 | Qdot 605 | lineage | T cell, T memory, Treg | High |
| CD45RA | Human | 5H9 | Qdot 705 | activation/differentiation | T cell, T memory | Med |
| CD8a | Human | RPA-T8 | Qdot 585 | lineage | T cell, T memory | High |
| Dead cells | Human | Live/Dead Fix Aqua | viability | |||
| CD25 | Human | M-A251 | PE-Cy5 | Treg | T cell, Treg | Low |
| CD38 | Human | HIT2 | PE-CF594 | activation/differentiation | T cell, activation | Low |
| CD45RO | Human | UCHL1 | FITC | activation/differentiation | T cell, T memory | Med |
| CD73 | Human | AD2 | PE | Treg function | T cell, Treg | Med |
| CD127 | Human | eBioRDR5 | APC-eFluor 780 | Treg | T cell, Treg | Low |
| PD-1 | Human | EH12.2H7 | Brilliant Violet 421 | activation/differentiation | T cell, activation | Low |
| HLA DR | Human | G46-6 | Alexa Fluor 680 | activation/differentiation | T cell, activation | High |
| CD39 | Human | eBioA1 (A1) | PE-Cy7 | Treg function | T cell, activation, Treg, T memory | Low |
