OMIP-014
Validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining
Cytometry PART A, Volume 81A, Issue 5, 362-363 (2012)
Stephen C. De Rosa, Donald K. Carter, M. Juliana McElrath
Description: This panel was developed, optimized, and validated for assessment of CD4+ and CD8+ T-cell responses to various peptide pools for antigens of interest in cryopreserved peripheral blood mononuclear cells (PBMC) from adult humans. The panel has been used to evaluate HIV- and TB-specific responses to candidate vaccines for these pathogens, although the panel can be used with peptide pools for any proteins. The panel has not been tested with freshly-isolated PBMC or with whole blood.
Species: Human
Tissue: Peripheral blood
Cell Subset: T cell, T helper, Ctl
Keyword: Antigen-specific t cell response, Cryopreserved pbmc, Hiv, Lysed whole blood (lwb), Tuberculosis (tb), Vaccination
Machine: BD LSR2
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| TNF | Human | Mab11 | FITC | function | T cell, T helper, CTL | High |
| Dead cells | Human | Live/Dead Fix Aqua | viability | |||
| CD14 | Human | Qdot 655 | exclusion | High | ||
| IFN-gamma | Human | B27 | V450 | function | T cell, T helper, CTL | High |
| IL-4 | Human | MP4-25D2 | APC | function | T cell, T helper | Low |
| CD107a | Human | H4A3 | PE-Cy7 | function | T cell, activation, T helper, CTL | Low |
| CD3 | Human | UCHT1 | PE-Texas Red | lineage | T cell, T helper, CTL | High |
| CD4 | Human | 13B8.2 | APC-Alexa 750 | lineage | T cell, T helper | High |
| CD40L | Human | TRAP1 (also known as TRAP-1) | PE-Cy5 | function | T cell, T helper | Low |
| CD8a | Human | SK1 | PerCP-Cy5.5 | lineage | T cell, CTL | High |
| IL-2 | Human | MQ1-17H12 | PE | function | T cell, T helper, CTL | Med |
| MIP-1 beta | Human | D21-1351 | Alexa Fluor 700 | function | T cell, CTL, activation, T helper | Med |
