OMIP-013
Differentiation of human T-cells
Cytometry PART A, Volume 81A, Issue 11, 935-936 (2012)
Yolanda D. Mahnke, Margaret H. Beddall, Mario Roederer
Description: The present panel was optimized to investigate the differentiation status of CD4+ and CD8+ T-cells in peripheral blood mononuclear cells (PBMC) from healthy individuals. It works well with cryopreserved PBMC and we have observed similar results with fresh specimens. Other tissue types have not been tested.
Species: Human
Tissue: Peripheral blood
Cell Subset: T cell, T memory, Recent thymic emigrants (rte)
Keyword: Cryopreserved pbmc
Machine: BD LSR2
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD244 | Human | C1.7 | PE-Cy5.5 | memory/differentiation (exploratory) | T cell | Med |
| CD4 | Human | OKT4 | Qdot 605 | T-cell subset | T cell, T memory, Tscm | High |
| CD57 | Human | NK-1 | Qdot 705 | memory/differentiation for TSCM | T cell, Tscm | High |
| Dead cells | Human | Live/Dead Fix Aqua | viability | |||
| CD27 | Human | O323 | FITC | memory/differentiation | T cell, T memory, Tcm | Med |
| CD8a | Human | RPA-T8 | Qdot 585 | T-cell subset | T cell, T memory | High |
| CD3 | Human | SK7 | APC-H7 | T-cell subset | T cell, T memory, Tscm | High |
| CD45RA | Human | HI100 | APC | memory/differentiation | T cell, T memory | Med |
| CD127 | Human | A019D5 | Brilliant Violet 421 | memory/differentiation for TSCM | T cell, Tscm | Med |
| CCR7 | Human | 150503 | Alexa Fluor 680 | memory/differentiation | T cell, T memory, Tcm, T naive | Low |
| CD95 | Human | Dx2 | PE | memory/differentiation for RTE and TSCM | T cell, Tscm, RTE | Low |
| CD31 | Human | WM59 | PE-Cy7 | memory/differentiation for RTE and TSCM | T cell, T memory, Tscm, RTE | High |
| CD28 | Human | CD28.2 | PE-Cy5 | memory/differentiation for TSCM | T cell, T memory, Tscm | Med |
