OMIP-098
A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotyping
https://doi.org/10.1002/cyto.a.24802
Matthew Creegan, Justin Degler, Dominic Paquin-Proulx, Michael A. Eller, Kawthar Machmach
Description: This 26-parameter flow cytometry panel has been developed and optimized to analyze NK cell phenotype, using cryopreserved peripheral blood mononuclear cells (PBMCs) from people living with and without human immunodeficiency virus (PLWH, PWOH). Our panel is designed for the analysis of several parameters of total NK cells and memory NK cell subsets including markers of maturation, activation, and proliferation, as well as activating and inhibitory receptors. Other tissues have not been tested.
Species: Human
Tissue: Peripheral blood
Cell Subset: Nk
Keyword: Activation, Primary immunodeficiency (pid), Proliferation
Machine: FACS Symphony
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD159a (NKG2A) | Human | REA110 | PE-Vio770 | Inhibitory receptors | NK | Low |
| CD33 | Human | WM53 (also known as WM-53) | Brilliant Violet 786 | Non-NK cell lineage exclusion | Med | |
| CD19 | Human | SJ25C1 (also known as SJ25-C1) | Brilliant Violet 786 | Non-NK cell lineage exclusion | Low | |
| KLRG1 | Human | SA231A2 | Brilliant Violet 421 | Inhibitory receptors | NK | Low |
| CD159c | Human | REA205 | PE-Vio615 | Activating receptors | NK, NK memory | Low |
| CD337 | Human | p30-15 | Red 718 | Activating receptors | NK | Low |
| NKp80 | Human | 5D12 | Brilliant Violet 650 | Activating receptors | NK | Med |
| Fc epsilon R1 gamma | Human | polyclonal | FITC | NK Cell subset | NK, NK memory | Low |
| CD158e1 (KIR3DL1) | Human | DX9 | PerCP-Cy5.5 | Inhibitory receptors | NK | High |
| CD94 | Human | HP-3D9 | Brilliant Ultraviolet 805 | Activating/inhibitory receptors | NK | Med |
| CD57 | Human | NK-1 | Brilliant Ultraviolet 395 | Maturation marker | NK, NK memory | High |
| PD-1 | Human | EH12.1 (also known as EH12) | Brilliant Ultraviolet 661 | Exhaustion marker | NK | Low |
| Live/Dead Fix Aqua | Human | Live/Dead Fix Aqua | Viability marker | |||
| CD3 | Human | UCHT1 (also known as UCHT-1; UCHT 1) | Brilliant Violet 786 | Non-NK cell lineage exclusion | High | |
| CD16 | Human | 3G8 | Brilliant Ultraviolet 496 | NK cell subset | NK | Med |
| CD56 | Human | B159 | Brilliant Ultraviolet 563 | NK Cell subset | NK, NK memory | Low |
| CD158 | Human | HP-MA4 | PerCP-Cy5.5 | Inhibitory receptors | NK | Med |
| CD328 | Human | REA214 | APC-Vio770 | Inhibitory receptors | NK | High |
| CD85j | Human | GHI/75 | Brilliant Ultraviolet 615 | Inhibitory receptors | NK | Low |
| CD314 (NKG2D) | Human | 1D11 | Brilliant Blue 790-P | Activating receptors | NK | Low |
| NKp46 | Human | 9E2/NKp46 (also known as 9-E2) | Brilliant Violet 711 | Activating receptors | NK | Low |
| HLA DR | Human | G46-6 (also known as L243) | Brilliant Violet 480 | Activation marker | NK | High |
| CD38 | Human | HB7 (also known as HB-7) | Brilliant Ultraviolet 737 | Activation marker | NK | Low |
| Ki-67 | Human | B56 | Brilliant Violet 750 | Proliferation marker | Unspecified | |
| EOMES | Human | WD1928 | PE | Transcription factor | NK | Low |
| T-bet | Human | 4B10 | PE-Cy5 | Transcription factor | NK | Low |
| Integrin alpha 4 beta 7 | Human | 4B10 | Alexa Fluor 647 | Homing marker | NK | Low |
