| OMIP-081 |
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|---|---|---|---|---|---|---|---|
A new 21-monoclonal antibody 10-color panel for diagnostic polychromatic immunophenotyping |
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| Erik H. L. P. G. Huys,Willemijn Hobo,Frank W. M. B. Preijers TBD | |||||||
PURPOSE: The 10-color panel consisting of 21 monoclonal antibodies (mAbs) is developed as a one-tube panel to detect leukemia and lymphoma cells in all hematopoietic cell lineages. In particular, this tube is mentioned for a fast screening to identify aberrant cells in samples suspected for malignant cell localization and to enable comprehensive immunophenotyping of samples with low cell counts.
CELL TYPE: Bone marrow, peripheral blood
MACHINE: Beckman Coulter Navios
| Marker | Target | Clone | Fluorochrome | Purpose |
|---|---|---|---|---|
| CD45 | Human | J33 | Krome Orange | WBC subpopulations |
| CD3 | Human | UCHT1 | APC-Alexa 750 | Mature T cells |
| CD4 | Human | SFCI12T4D11 | PE-Cy7 | Helper T cells, Monocyte lineage |
| CD8 | Human | B9.11 | Pacific Blue | Cytotoxic T cells, NK-cell subset |
| CD2 | Human | APC-Alexa 700 | T-cell lineage, NK-cell subset | |
| CD5 | Human | BL1a | PE-Cy5.5 | T-cell lineage, B-cell subset |
| CD7 | Human | 8H8.1 | FITC | T-cell lineage, T-cell activation, NK-cell subsets |
| CD16 | Human | B73.1 | PE | T-cell subsets, NK cells, monocyte differentiation, eosinophil exclusion, myeloid lineage |
| CD56 | Human | NCAM16.2 | PE | NK cells, plasma cell subset |
| CD19 | Human | ECD | B-cell lineage | |
| CD20 | Human | B9E9 | Pacific Blue | Mature B cells |
| Ig Kappa Light Chain | Human | APC | B-cell subset, B-cell lineage clonality | |
| Ig Lambda Light Chain | Human | PE | B-cell subset, B-cell lineage clonality | |
| CD138 | Human | B-B4 | FITC | Plasma cells |
| CD14 | RMO52 | ECD | Mature monocytes | |
| CD36 | Human | FA6.152 | APC-Alexa 700 | Monocyte lineage, erythroid lineage, megakaryocytes |
| CD33 | Human | D3HL60.251 | PE-Cy5.5 | Monocyte lineage, myeloid lineage |
| CD15 | Human | 80H5 | Pacific Blue | Myeloid lineage |
| CD34 | Human | 581 | PE-Cy7 | Progenitor cells |
| CD117 | Human | 104D2D1 | APC | Myeloid precursors |
| CD10 | Human | ALB1 | APC-Alexa 700 | B-cell precursors, mature neutrophils |
