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29-Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantation

Sarka Vanikova,Abhishek Koladiya,Jan Musil TBD

PURPOSE: This 29-color panel was developed and optimized for the monitoring of NK cell and T cell reconstitution in peripheral blood of patients after HSCT.



Marker Target Clone Fluorochrome Purpose
CD62L Human DREG-56 Brilliant Ultraviolet 395 T cell differentiation
Live/Dead Fix Blue All Species Live/Dead Fix Blue Viability
CD69 Human FN50 Brilliant Ultraviolet 496 NK and T cell activation marker
CCR6 Human 11A9 Brilliant Ultraviolet 563 Chemokine receptor; Th subset identification
CD27 Human M-T271 Brilliant Ultraviolet 615 T cell differentiation
PD-1 Human EH12.1 Brilliant Ultraviolet 661 T cell inhibitory receptor
CD25 Human 2A3 Brilliant Ultraviolet 737 T cell activation; Treg identification
CD8 Human RPA-T8 Brilliant Ultraviolet 805 CD8 T cell and NKT-like cell lineage marker
KLRC1 Human 131411 Brilliant Violet 421 NK and NKT-like cell inhibitory receptor
CD45RA Human HI100 Pacific Blue T cell differentiation
TIM-3 Human 7D3 Brilliant Violet 480 T cell inhibitory receptor
CD4 Human RPA-T4 Brilliant Violet 570 CD4 T cell lineage marker
CD57 Human QA17A04 Brilliant Violet 605 T cell and NK cell differentiation
CD95 Human DX2 Brilliant Violet 650 T cell activation and differentiation
TCR gamma/delta Human 11F2 Brilliant Violet 711 γδ T cells
CD226 Human DX11 Brilliant Violet 750 T cell and NK cell activating receptor
CD31 Human WM59 Brilliant Violet 786 Adhesion molecule; identification of RTE
CCR10 Human 1B5 Brilliant Blue 515 Chemokine receptor; Th subset identification
CD194 Human [1G1] Brilliant Blue 700 Chemokine receptor; Th subset identification
TIGIT Human 741182 Brilliant Blue 755-P T cell and NK cell inhibitory receptor
CD314 Human 1D11 Brilliant Blue 790-P NK cell activating receptor
KLRC2 Human 134591 PE NK cell activating receptor
CD56 Human NCAM16.2 PE-CF594 NK cell and NKT-like cell lineage marker
CD183 Human 1C6/CXCR3 PE-Cy5 Chemokine receptor; Th subset identification
FoxP3 Human PCH101 PE-Cy5.5 Master transcription factor for Tregs
CD39 Human PE-Cy7 Treg activation marker
CD3 Human UCHT1 Alexa Fluor 647 T cell and NKT-like cell lineage marker
CD16 Human 3G8 Alexa Fluor 700 NK cell differentiation
Perforin Human B-D48 APC-Fire 750 Cytolytic function