| OMIP-080 |
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|---|---|---|---|---|---|---|---|
29-Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantation |
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| Sarka Vanikova,Abhishek Koladiya,Jan Musil TBD | |||||||
PURPOSE: This 29-color panel was developed and optimized for the monitoring of NK cell and T cell reconstitution in peripheral blood of patients after HSCT.
CELL TYPE: Human PBMCs
MACHINE: BD FACSymphony A5
| Marker | Target | Clone | Fluorochrome | Purpose |
|---|---|---|---|---|
| CD62L | Human | DREG-56 | Brilliant Ultraviolet 395 | T cell differentiation |
| Live/Dead Fix Blue | All Species | Live/Dead Fix Blue | Viability | |
| CD69 | Human | FN50 | Brilliant Ultraviolet 496 | NK and T cell activation marker |
| CCR6 | Human | 11A9 | Brilliant Ultraviolet 563 | Chemokine receptor; Th subset identification |
| CD27 | Human | M-T271 | Brilliant Ultraviolet 615 | T cell differentiation |
| PD-1 | Human | EH12.1 | Brilliant Ultraviolet 661 | T cell inhibitory receptor |
| CD25 | Human | 2A3 | Brilliant Ultraviolet 737 | T cell activation; Treg identification |
| CD8 | Human | RPA-T8 | Brilliant Ultraviolet 805 | CD8 T cell and NKT-like cell lineage marker |
| KLRC1 | Human | 131411 | Brilliant Violet 421 | NK and NKT-like cell inhibitory receptor |
| CD45RA | Human | HI100 | Pacific Blue | T cell differentiation |
| TIM-3 | Human | 7D3 | Brilliant Violet 480 | T cell inhibitory receptor |
| CD4 | Human | RPA-T4 | Brilliant Violet 570 | CD4 T cell lineage marker |
| CD57 | Human | QA17A04 | Brilliant Violet 605 | T cell and NK cell differentiation |
| CD95 | Human | DX2 | Brilliant Violet 650 | T cell activation and differentiation |
| TCR gamma/delta | Human | 11F2 | Brilliant Violet 711 | γδ T cells |
| CD226 | Human | DX11 | Brilliant Violet 750 | T cell and NK cell activating receptor |
| CD31 | Human | WM59 | Brilliant Violet 786 | Adhesion molecule; identification of RTE |
| CCR10 | Human | 1B5 | Brilliant Blue 515 | Chemokine receptor; Th subset identification |
| CD194 | Human | [1G1] | Brilliant Blue 700 | Chemokine receptor; Th subset identification |
| TIGIT | Human | 741182 | Brilliant Blue 755-P | T cell and NK cell inhibitory receptor |
| CD314 | Human | 1D11 | Brilliant Blue 790-P | NK cell activating receptor |
| KLRC2 | Human | 134591 | PE | NK cell activating receptor |
| CD56 | Human | NCAM16.2 | PE-CF594 | NK cell and NKT-like cell lineage marker |
| CD183 | Human | 1C6/CXCR3 | PE-Cy5 | Chemokine receptor; Th subset identification |
| FoxP3 | Human | PCH101 | PE-Cy5.5 | Master transcription factor for Tregs |
| CD39 | Human | PE-Cy7 | Treg activation marker | |
| CD3 | Human | UCHT1 | Alexa Fluor 647 | T cell and NKT-like cell lineage marker |
| CD16 | Human | 3G8 | Alexa Fluor 700 | NK cell differentiation |
| Perforin | Human | B-D48 | APC-Fire 750 | Cytolytic function |
