Description: The present optimized multicolor immunofluorescence panel (OMIP) provides for the detection and in-depth analysis of all major leukocyte subsets in human whole blood (WB) using a single flow cytometry (FCM) panel with just 14 colors. The panel offers an easy, fast and reproducible way of comprehensive immune cell profiling for various purposes including translational research and companion immune monitoring of clinical trials. Of note, the panel has been specifically designed to analyze erythrocyte-depleted WB, hence allowing the characterization of polymorphonuclear cells in addition to the widely used peripheral blood mononuclear cells (PBMCs). The use of WB instead of density gradient-enriched material further permits absolute cell counting in the original sample volume. Other notable assets are the detection of hematopoietic progenitors as well as the possibility for myeloid dendritic cell subset discrimination. In contrast to high-dimensional FCM or mass cytometry (MCM) panels, this 14-color panel does not require high-end instrumentation and is therefore accessible to a particularly broad research community.

Species: Human

Tissue: Peripheral blood

Cell Subset: B cell, T cell, Dc, Mdc/cdc, Pdc, Nk, Monocyte, Basophil, Eosinophil, Neutrophil, Progenitors/ hsc, Mdc1

Keyword: Immunoprofiling, Lysed whole blood (lwb)

Machine: BD LSR Fortessa