| OMIP-011 |
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Characterization of circulating endothelial cells (CECs) in peripheral blood |
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| Raskit Lachmann, Paola Lanuti, Sebastiano Miscia Cytometry PART A, Volume 81A, Issue 7, 549-551 (2012) | |||||||
PURPOSE: This panel was optimized for the evaluation of circulating endothelial cells (CECs) in peripheral blood (see Table 1). The combination of three different endothelial cell markers enables a reasonable analysis of CECs. The panel, so far tested on fresh human peripheral and cord blood, can be used to enumerate CECs in a dual platform method.
CELL TYPE: Whole blood, RBC-lysed and washed
MACHINE: BD FACSCanto 2
| Marker | Target | Clone | Fluorochrome | Purpose |
|---|---|---|---|---|
| Dead cells | Human | Nile Red | Viability | |
| CD45 | Human | HI30 | Alexa Fluor 700 | Exclusion of leukocyte |
| DNA | Human | Syto 16 | DNA marker | |
| CD31 | Human | M89D3 | Alexa Fluor 647 | endothelial cell marker |
| CD34 | Human | 581 | ECD | endothelial cell marker |
| CD146 | Human | P1H12 | PE | endothelial cell marker |
| CD117 | Human | 104D2 | PE-Cy7 | progenitor marker |
| CD106 | Human | 51-10C9 | PE-Cy5 | activation |
