< OMIPS Directory

Characterization of circulating endothelial cells (CECs) in peripheral blood

Raskit Lachmann, Paola Lanuti, Sebastiano Miscia Cytometry PART A, Volume 81A, Issue 7, 549-551 (2012)

PURPOSE: This panel was optimized for the evaluation of circulating endothelial cells (CECs) in peripheral blood (see Table 1). The combination of three different endothelial cell markers enables a reasonable analysis of CECs. The panel, so far tested on fresh human peripheral and cord blood, can be used to enumerate CECs in a dual platform method.

CELL TYPE: Whole blood, RBC-lysed and washed


Marker Target Clone Fluorochrome Purpose
Dead cells Human Nile Red Viability
CD45 Human HI30 Alexa Fluor 700 Exclusion of leukocyte
DNA Human Syto 16 DNA marker
CD31 Human M89D3 Alexa Fluor 647 endothelial cell marker
CD34 Human 581 ECD endothelial cell marker
CD146 Human P1H12 PE endothelial cell marker
CD117 Human 104D2 PE-Cy7 progenitor marker
CD106 Human 51-10C9 PE-Cy5 activation