OMIP-074
Phenotypic analysis of IgG and IgA subclasses on human B cells
Cytometry PART A, Volume 99, Issue 9, 880-883 (2021)
Leonard Nettey, Reid Ballard, Thomas Liechti, Rosemarie D. Mason
Description: This panel was designed and optimized to characterize the phenotypic diversity of circulating human memory B cells with an emphasis on discriminating IgA and IgG subclasses. Markers to detect all human immunoglobulin isotypes (IgA, IgD, IgE, IgG, and IgM), sub-classes (IgA1, IgA2, IgG1, IgG2, IgG3, and IgG4), and light chains(Ig Kappa and Ig Lambda) were used in combination with markers to more precisely delineate memory B cell populations based on CD21and CD27. This panel has been tested on fresh and cryopreserved peripheral blood mononuclear cells (PBMC) from multiple healthy subjects.
Species: Human
Tissue: Peripheral blood
Cell Subset: Plasma cell/ asc, B cell, B memory, Double negative b, Follicular b cell, Switched b, Transitional b, Unswitched b
Keyword: Cryopreserved pbmc
Machine: FACSymphony A5
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD19 | Human | HIB19 | PE-Dazzle 594 | Lineage | naive, IgA, IgG, memory, light chain | Low |
| CD21 | Human | B-ly4 | Brilliant Violet 605 | B cell Subset | naive, memory | Med |
| IgE | Human | MHE-18 | APC-Fire 750 | Immunoglobulin Isotype | memory | High |
| IgG | Human | G18-145 | PE-Cy5 | Immunoglobulin Isotype | IgG | High |
| IgM | Human | MHM-88 | Brilliant Violet 570 | Immunoglobulin Isotype | naive | Med |
| IgG1 | Human | HP6001 | PE | Immunoglobulin subclass | IgG | Med |
| IG Kappa Light Chain | Human | G20-193 | Brilliant Violet 711 | Immunoglobulin light chain | light chain | High |
| Live/Dead Fix Aqua | Human | Live/Dead Fix Aqua | Viability | |||
| IgG3 | Human | HP6050 | Alexa Fluor 488 | Immunoglobulin subclass | IgG | Med |
| IgG4 | Human | SAG4 | APC | Immunoglobulin subclass | IgG | Med |
| IgG2 | Human | HP6002 | Immunoglobulin subclass | IgG | Med | |
| IgA | Human | polyclonal | DyLight 405 | Immunoglobulin Isotype | IgA | High |
| Streptavidin | Human | Brilliant Violet 750 | detect IgG2 | IgG | ||
| IgD | Human | IADB6 (IA6-2) | PE-Cy5.5 | Immunoglobulin subtype | Naive | High |
| CD20 | Human | 2H7 | Alexa Fluor 700 | B-Cell Subset | naive, IgA, IgG, memory, light chain | Med |
| IgA2 | Human | IS11-21E11 | PE-Vio770 | Immunoglobin subclass | IgA | Med |
| IgA1 | Human | B3506B4 | PerCP-Cy5.5 | Immunoglobulin subclass | IgA | Med |
| Ig Lambda Light Chain | Human | JDC-12 | Brilliant Violet 786 | Immunoglobulin light chain | light chain | Med |
| CD27 | Human | O323 | Brilliant Violet 650 | B cell subset | memory | Med |
