OMIPs Directory

OMIP-001

Quality and phenotype of Ag-responsive human T-cells

Mahnke YD, Roederer M. OMIP-001: Quality and phenotype of Ag-responsive human T-cells. Cytometry A. 2010;77(9):819-820. doi:10.1002/cyto.a.20944
Yolanda D. Mahnke, Mario Roederer

Description: The present panel was optimized for the evaluation of CD4+ and CD8+ T-cell responses to various HIV-1–derived peptide pools in peripheral blood mononuclear cells (PBMC) from HIV-1+ individuals with differences in clinical progression. It works well with cryopreserved PBMC, and we have observed similar results with fresh specimens. Other tissue types have not been tested.

Species: Human

Tissue: Peripheral blood

Cell Subset: T cell, T helper, T memory, Ctl

Keyword: Cryopreserved pbmc, Hiv

Machine: LSR II

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
CD4 Human M-T477 Qdot 605 lineage T cell, T memory High
CD19 Human HIB19 Pacific Blue exclusion Low
Biotin Human Streptavidin Qdot 655 secondary reagent to PD-1 T cell Low
CCR7 Human 150503 Alexa Fluor 680 T cell memory subsets T cell, T memory Low
Dead cells Human Live/Dead Fix Violet viability
CD45RO Human UCHL1 Qdot 545 T cell memory subsets T cell, T memory High
CD57 Human NK-1 Qdot 705 T cell function T cell High
CD27 Human M-T271 PE-Cy7 T cell memory subsets T cell, T memory Medium
CD8a Human RPA-T8 Qdot 585 lineage T cell High
PD-1 Human MIH4 memory/differentiation T cell Low
LT-alpha Human Mab11 Alexa Fluor 594 function T cell, T helper Medium
CD14 Human M5E2 Pacific Blue exclusion High
CD127 Human R34.34 PE T cell memory subsets T cell, T memory Medium
IFN-gamma Human B27 APC T cell function, T helper subsets T cell, T helper High
CD28 Human CD28.2 PE-Cy5 T cell memory subsets T cell, T memory Medium
IL-2 Human MQ1-17H12 Alexa Fluor 488 T cell function, T helper subsets T cell, T helper Medium
CD3 Human SK7 APC-Cy7 lineage T memory, T cell, T helper High