On-Chip Microfluidic cell Sorter Configuration

	Laser (nm)	LP	Optimal Colors Additional optimal fluorochrome options available on FluoroFinder.com.
Laser	405	445 / 20	--	AbBy Fluor 405 ABflo 405 ABflo 450 Aco-490 Alexa Fluor 405 CF405M CF405S cFluor V450 CoraLite Plus 405 CytoCalcein Violet 450 DY-415 DyLight 405 eFluor 450 ExoBrite 410/450 EV Membrane Stain HiLyte 405 iFluor 405 LysoView 405 MaxLight 405 MemBrite Fix 405/430 mFluor Violet 450 MitoLite Blue FX490 MitoView 405 Nuclear Violet Oxazole Blue Homodimer Pacific Blue POPO-1 PromoFluor 405 RayBright Violet 450 Spark Violet 423 StarBright Violet 440 SuperNova v428 V450 Vio Blue Vio Bright V423 violetFluor 450
		543 / 22	--	ATTO 430LS Brilliant Violet 510 CytoCalcein Violet 500 DY-395XL LipidSpot 488 LysoSensor Yellow/Blue pH3.0 MitoLite Orange EX405 Nuclear Yellow Spark Violet 538 StarBright Violet 570 Vio Green
		592 / 40	--	ABflo 610 ATTO 430LS Brilliant Violet 605 cFluor V610 DY-395XL LipidSpot 488 LysoSensor Yellow/Blue pH3.0 mFluor Violet 610 Spark Violet 538 StarBright Violet 570 StarBright Violet 610 SuperBright 600 SuperNova v605 Vio Bright V600
		607 / 24	--	ABflo 610 ATTO 430LS Brilliant Violet 605 cFluor V610 DY-395XL LipidSpot 488 LysoSensor Yellow/Blue pH3.0 mFluor Violet 610 StarBright Violet 570 StarBright Violet 610 SuperBright 600 SuperNova v605 Vio Bright V600
		676 / 37	--	Brilliant Violet 650 Brilliant Violet 711 Chlorophyll A StarBright Violet 670 SuperBright 645 SuperBright 702 eFluor 650NC eVolve 655 Qdot 655 Qdot 705
		--	710	Aco-800 Brilliant Violet 711 Brilliant Violet 750 Brilliant Violet 785 Brilliant Violet 786 StarBright Violet 710 StarBright Violet 760 StarBright Violet 790 SuperBright 702 SuperBright 780 SuperNova v786

Laser	488	543 / 22	--	AbBy Fluor 488 ABflo 488 Adirondack Green 520 Alexa Fluor 488 Alpha Fluor 488 ATTO 488 BactoView Dead 500/515 BactoView Live Green BCECF AM Brilliant Blue 515 CellTrace Oregon Green CellTracker Green CF488A cFluor B515 cFluor B532 cFluor B548 Chromeo 488 Chromeo 505 CoraLite 532 CoraLite Plus 488 CytoTrace Green CMFDA DAF-FM DMAO DyLight 488 ExoBrite 490/515 EV Membrane Stain FITC FITC Plus HiLyte 488 iFluor 488 KIRAVIA Blue 520 LysoView 488 MaxLight 490 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoMark Green MitoROS 520 NBD NL 493 NovaFluor Blue 530 Nuclear Green Nuclear Orange NucSpot 470 NucSpot Live 488 Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 512 Nucleic Acid Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator PicoGreen PromoFluor 488P RayBright Blue 488 RealBlue 545 Spark Blue 550 Spark Blue 574 Spectrum Green SureLight 488 Syto 16 Syto 24 Thiazole Orange Homodimer Thiazole Orange Iodide Vio 488 Vio 515 Vio Bright 515 Vio Bright FITC VRDye 490 XFD488 Y-PE
		592 / 40	--	Aco-600 Alexa Fluor 555 cFluor B548 cFluor BYG575 cFluor BYG610 Chromeo 494 CoraLite 532 Di-8-ANEPPS NBD NovaFluor Blue 610 / 30S Nuclear Orange NucSpot 470 PE PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue 512 Nucleic Acid Stain RayBright PE RealBlue 613 SNARF 1 pH6.0 Spark Blue 574 StarBright Blue 580 StarBright Blue 615 SureLight PE-594 Thiazole Orange Homodimer Thiazole Orange Iodide Y-PE
		607 / 24	--	Aco-600 ATTO 490LS cFluor BYG610 Chromeo 494 Di-8-ANEPPS NovaFluor Blue 610 / 30S Nuclear Orange NucSpot 470 PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue 512 Nucleic Acid Stain RealBlue 613 SNARF 1 pH6.0 Spark Blue 574 StarBright Blue 580 StarBright Blue 615 SureLight PE-594
		676 / 37	--	Aco-600 ATTO 490LS Brilliant Blue 700 cFluor B690 cFluor BYG667 cFluor BYG680 Chromeo 494 Di-8-ANEPPS NovaFluor Blue 660 / 40S NovaFluor Blue 690 PE-Cy5 PE-Cy5.5 PE-Dyomics 647 PE-Fire 640 PE-Fire 700 PerCP-eFluor 710 PerCP-Vio700 PE-Vio670 RealBlue 705 RPE-CF647T StarBright Blue 675 StarBright Blue 700 SureLight PE Cyanine 5.0 SureLight PE Cyanine 5.5
		--	710	ATTO 490LS Brilliant Blue 700 cFluor BYG710 cFluor BYG781 Di-8-ANEPPS NovaFluor Blue 690 NovaFluor Blue 760 PE-Cy7 PE-Fire 700 PE-Fire 810 PerCP-eFluor 710 PerCP-Fire 806 PerCP-Vio700 PE-Vio770 RayBright PE-Cy7 RealBlue 705 RealBlue 744 RealBlue 780 Seta-R-PE-775 StarBright Blue 700 StarBright Blue 765 StarBright Blue 810 SureLight PE Cyanine 7.0

Laser	561	592 / 40	--	AbBy Fluor 555 ABflo 555 Aco-600 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 561 ATTO 542 ATTO 550 ATTO 565 ATTO Rho3B BOBO-3 Calcein Red AM Calcein Red-Orange AM Calcium Orange CellMask Orange Plasma Membrane Stain CF568 CF570 cFluor BYG575 cFluor BYG610 cFluor YG584 cFluor YG610 CytoTrace Red DyLight 549 DyLight 550 Dyomics 547 ExoBrite 560/585 EV Membrane Stain HiLyte 555 LysoBrite Orange LysoBrite Red LysoTracker Red LysoView 550 LysoView 594 Magic Red MemBrite Fix 568/580 mFluor Blue 570 MitoLite Orange FX570 MitoLite Red FX600 MitoMark Red mNeptune mScarlet NL 557 NovaFluor Yellow 570 NovaFluor Yellow 610 PE PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue 551 Mitochondrial Stain PhenoVue 577 Lysosomal Stain PhenoVue 578 Mitochondrial Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Fluor 555 PhenoVue Nile Red Lipid Stain PhenoVue ROS-570, Total Oxidative Stress Indicator RayBright PE RealYellow 586 RealYellow 610 ReAsH Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 SNARF 1 pH9.0 Spark YG 581 StarBright Yellow 605 SureLight PE-594 XFD555
		607 / 24	--	Aco-600 ATTO 550 ATTO 565 ATTO Rho3B BOBO-3 Calcein Red-Orange AM Calcium Orange CellMask Orange Plasma Membrane Stain CF568 CF570 cFluor BYG610 cFluor YG584 cFluor YG610 DyLight 549 Dyomics 547 ExoBrite 560/585 EV Membrane Stain LysoBrite Red LysoTracker Red LysoView 550 LysoView 594 Magic Red MemBrite Fix 568/580 MitoLite Orange FX570 MitoLite Red FX600 MitoMark Red mNeptune mScarlet NovaFluor Yellow 610 PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue 551 Mitochondrial Stain PhenoVue 577 Lysosomal Stain PhenoVue 578 Mitochondrial Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Nile Red Lipid Stain RealYellow 586 RealYellow 610 ReAsH Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 SNARF 1 pH9.0 Spark YG 581 StarBright Yellow 605 SureLight PE-594
		676 / 37	--	Aco-600 BactoView Live Red cFluor BYG667 cFluor BYG680 DRAQ 9 LipidSpot 610 LysoView 594 mNeptune NovaFluor Yellow 660 NucSpot Far-Red PE-Cy5 PE-Cy5.5 PE-Dyomics 647 PE-Fire 640 PE-Fire 700 PE-Vio670 PhenoVue Nile Red Lipid Stain PromoFluor 633P RealYellow 703 RPE-CF647T SNARF 1 pH9.0 StarBright Yellow 665 SureLight P3 SureLight PE Cyanine 5.0 SureLight PE Cyanine 5.5 SureLight RPC
		--	710	BactoView Live Red cFluor BYG710 cFluor BYG781 DRAQ 9 LipidSpot 610 NovaFluor Yellow 755 NucSpot Far-Red PE-Cy7 PE-Fire 700 PE-Fire 810 PE-Vio770 RayBright PE-Cy7 RealYellow 703 RealYellow 775 Seta-R-PE-775 StarBright Yellow 720 StarBright Yellow 800 SureLight PE Cyanine 7.0

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.

NYU Langone Medical Center Cytometry and Cell Sorting Core

On-Chip Microfluidic cell Sorter Configuration

405

445 / 20

--

543 / 22

--

592 / 40

--

607 / 24

--

676 / 37

--

--

710

488

543 / 22

--

592 / 40

--

607 / 24

--

676 / 37

--

--

710

561

592 / 40

--

607 / 24

--

676 / 37

--

--

710

NYU Langone Medical Center
Cytometry and Cell Sorting Core