FACS Canto Analyzer Configuration

	Laser (nm)	LP	Optimal Colors Additional optimal fluorochrome options available on FluoroFinder.com.
Laser	488	530 / 40	502	AbBy Fluor 488 ABflo 488 Adirondack Green 520 Alexa Fluor 488 Alpha Fluor 488 ATTO 488 BactoView Dead 500/515 BactoView Live Green BCECF AM Brilliant Blue 515 CellTrace Oregon Green CellTracker Green CF488A CF500 cFluor B515 cFluor B532 cFluor B548 Chromeo 488 Chromeo 505 CoraLite 532 CoraLite Plus 488 CytoTrace Green CMFDA DAF-FM DMAO DyLight 488 ExoBrite 490/515 EV Membrane Stain FITC FITC Plus HiLyte 488 iFluor 488 KIRAVIA Blue 520 LysoBrite Green LysoView 488 MaxLight 490 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoMark Green MitoROS 520 NBD Neuro DiO NL 493 NovaFluor Blue 530 Nuclear Green Nuclear Orange NucSpot 470 NucSpot Live 488 Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue 512 Nucleic Acid Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator PicoGreen PromoFluor 488P RayBright Blue 488 RealBlue 545 Spark Blue 550 Spark Blue 574 Spectrum Green SureLight 488 Syto 16 Syto 24 Thiazole Orange Homodimer Thiazole Orange Iodide Vio 488 Vio 515 Vio Bright 515 Vio Bright FITC VRDye 490 XFD488 Y-PE
		586 / 15	556	Aco-600 Alexa Fluor 555 cFluor B548 cFluor BYG575 cFluor BYG610 Chromeo 494 CoraLite 532 Di-8-ANEPPS NBD Nuclear Orange NucSpot 470 PE PE-Dazzle 594 PE-Dyomics 590 PE-eFluor 610 PhenoVue 512 Nucleic Acid Stain RayBright PE SNARF 1 pH6.0 Spark Blue 574 StarBright Blue 580 StarBright Blue 615 SureLight PE-594
		695 / 40	600	ATTO 490LS Brilliant Blue 700 cFluor B690 cFluor BYG667 cFluor BYG680 cFluor BYG710 Chromeo 494 Di-8-ANEPPS NovaFluor Blue 660 / 40S NovaFluor Blue 690 PE-Cy5 PE-Cy5.5 PE-Dyomics 647 PE-Fire 700 PerCP-eFluor 710 PerCP-Vio700 PE-Vio670 RealBlue 705 RPE-CF647T StarBright Blue 675 StarBright Blue 700 SureLight PE Cyanine 5.0 SureLight PE Cyanine 5.5
		780 / 60	735	cFluor BYG781 NovaFluor Blue 760 PE-Cy7 PE-Fire 810 PerCP-Fire 806 PE-Vio770 RayBright PE-Cy7 RealBlue 744 RealBlue 780 Seta-R-PE-775 StarBright Blue 765 StarBright Blue 810 SureLight PE Cyanine 7.0

Laser	407	610 / 20	595	ABflo 610 Brilliant Violet 605 DY-395XL LipidSpot 488 mFluor Violet 610 StarBright Violet 610 SuperBright 600 SuperNova v605 Vio Bright V600 DyLight 405LS
		630 / 30	505	ABflo 610 Brilliant Violet 605 Brilliant Violet 650 DY-395XL LipidSpot 488 mFluor Violet 610 StarBright Violet 610 SuperBright 600 SuperBright 645 SuperNova v605 Vio Bright V600
		450 / 50	--	AbBy Fluor 405 ABflo 405 ABflo 450 Aco-490 Alexa Fluor 405 ATTO 425 Brilliant Violet 421 Brilliant Violet 480 CF405M CF405S CoraLite Plus 405 CytoCalcein Violet 450 CytoCalcein Violet 500 DY-415 DyLight 405 eFluor 450 ExoBrite 410/450 EV Membrane Stain HiLyte 405 iFluor 405 LysoBrite Blue LysoView 405 MaxLight 405 MemBrite Fix 405/430 mFluor Violet 450 MitoLite Blue FX490 Nuclear Violet Oxazole Blue Homodimer Pacific Blue POPO-1 PromoFluor 405 RayBright Violet 450 Spark Violet 423 Spectrum Aqua StarBright Violet 440 StarBright Violet 475 SuperBright 436 SuperNova v428 V450 Vio Blue Vio Bright V423 violetFluor 450

Laser	640	660 / 20	--	AbBy Fluor 647 ABflo 647 Alexa Fluor 633 Alexa Fluor 635 Alexa Fluor 647 Alexa Fluor 660 APC ATTO 633 ATTO 647 ATTO 647N ATTO 655 ATTO 665 Calcein Deep Red AM CellMask Deep Red Plasma Membrane Stain CellTracker Deep Red CellVue Claret CF633 CF640R CF647 cFluor R659 cFluor R668 cFluor R685 Chromeo 642 CoraLite 647A CoraLite Plus 647 Cy5 CypHer5E DiIC1(5) DRAQ 9 DyLight 633 DyLight 649 DyLight 650 Dyomics 647 ExoBrite 640/660 EV Membrane Stain FluoroProbes647h HiLyte 647 iFluor 633 iFluor 647 IRDye 650 Janelia Fluor 635 Janelia Fluor 646 Janelia Fluor 669 LipidSpot 610 LysoBrite NIR LysoTracker Deep Red LysoView 640 LysoView 650 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix 660/680 MemBrite Fix ST 650/665 MemBrite Fix ST 667/685 MitoLite Deep Red FX660 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NL 637 NucSpot 640 NucSpot Far-Red NucSpot Live 650 Oyster 650 PhenoVue 641 Mitochondrial Stain PhenoVue Fluor 647 PhenoVue ROS-670, Total Oxidative Stress Indicator PromoFluor 647P RayBright APC RayBright Red 647 Spark NIR 685 STAR 635 STAR 635P STAR RED SureLight APC SureLight P3 SureLight RPC Thiazole Red Thiazole Red Homodimer Vio 667 Vio Bright 667 Vio Bright R667 Vio R667 XFD647
		710 / 50	685	AbBy Fluor 647 Alexa Fluor 647 Alexa Fluor 660 APC-Alexa 680 APC-Alexa 700 APC-R700 ATTO 633 ATTO 647 ATTO 647N ATTO 655 ATTO 665 CellMask Deep Red Plasma Membrane Stain CellTracker Deep Red CellVue Claret cFluor R685 cFluor R720 CoraLite Plus 647 CypHer5E DiIC1(5) DRAQ 9 DyLight 649 Dyomics 647 FluoroProbes647h HiLyte 647 iFluor 647 IRDye 650 IRDye 680LT IRDye 680RD Janelia Fluor 669 LipidSpot 610 LysoView 640 LysoView 650 LysoView 680 MaxLight 650 MemBrite Fix 660/680 MemBrite Fix ST 667/685 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NovaFluor Red 725 NucSpot 640 NucSpot 680 NucSpot Far-Red NucSpot Live 650 Oyster 650 PhenoVue Fluor 647 PhenoVue ROS-670, Total Oxidative Stress Indicator PromoFluor 647P Red 718 Seta-APC-705 Spark NIR 685 STAR RED SureLight P3 Vio 667 Vio Bright 667 Vio Bright R667 Vio Bright R720 Vio R667 Vio R720 XFD647
		780 / 60	755	APC-CF750 APC-CF770 APC-eFluor 780 APC-Fire 750 APC-Fire 810 APC-H7 APC-Vio770 cFluor R780 cFluor R840 DRAQ 9 LysoView 680 NovaFluor Red 755 PE-Fire 780 RayBright Red 780 Seta-APC-780

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.

NYU Langone Medical Center Cytometry and Cell Sorting Core

FACS Canto Analyzer Configuration

488

530 / 40

502

586 / 15

556

695 / 40

600

780 / 60

735

407

610 / 20

595

630 / 30

505

450 / 50

--

640

660 / 20

--

710 / 50

685

780 / 60

755

NYU Langone Medical Center
Cytometry and Cell Sorting Core