NYU Langone Medical Center
Cytometry and Cell Sorting Core

Design a panel and find antibodies today at

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BD FACS Symphony A5 Analyzer Configuration

	Laser (nm)	LP	Optimal Colors Additional optimal fluorochrome options available on FluoroFinder.com.
Laser	355	379 / 28	--	Brilliant Ultraviolet 395
		450 / 50	410	Alexa Fluor 350 CF350
		515 / 30	450	Brilliant Ultraviolet 496
		586 / 15	550	Brilliant Ultraviolet 563
		670 / 30	635	Brilliant Ultraviolet 661
		740 / 35	690	Brilliant Ultraviolet 737
		820 / 60	770	Brilliant Ultraviolet 805

Laser	407	450 / 50	--	AbBy Fluor 405 ABflo 405 ABflo 450 Aco-490 Alexa Fluor 405 ATTO 425 Brilliant Violet 421 Brilliant Violet 480 CF405M CF405S CoraLite Plus 405 CytoCalcein Violet 450 CytoCalcein Violet 500 DY-415 DyLight 405 eFluor 450 ExoBrite 410/450 EV Membrane Stain HiLyte 405 iFluor 405 LysoBrite Blue LysoView 405 MaxLight 405 MemBrite Fix 405/430 mFluor Violet 450 MitoLite Blue FX490 Nuclear Violet Oxazole Blue Homodimer Pacific Blue POPO-1 PromoFluor 405 RayBright Violet 450 Spark Violet 423 Spectrum Aqua StarBright Violet 440 StarBright Violet 475 SuperBright 436 SuperNova v428 V450 Vio Blue Vio Bright V423 violetFluor 450
		474 / 25	450	ABflo 405 ABflo 450 Aco-490 ATTO 425 Brilliant Violet 480 Brilliant Violet 510 CF405M CF405S CoraLite Plus 405 CytoCalcein Violet 450 CytoCalcein Violet 500 DY-415 eFluor 450 ExoBrite 410/450 EV Membrane Stain HiLyte 405 iFluor 405 LysoBrite Blue LysoView 405 MemBrite Fix 405/430 mFluor Violet 450 MitoLite Blue FX490 Nuclear Violet Oxazole Blue Homodimer Pacific Blue POPO-1 RayBright Violet 450 Spectrum Aqua StarBright Violet 475 V450 Vio Blue Vio Green violetFluor 450
		586 / 15	550	ATTO 430LS Brilliant Violet 605 DY-395XL LipidSpot 488 LysoSensor Yellow/Blue pH3.0 Spark Violet 538 StarBright Violet 570 StarBright Violet 610 SuperBright 600 Vio Bright V600
		610 / 20	600	ABflo 610 Brilliant Violet 605 DY-395XL LipidSpot 488 mFluor Violet 610 StarBright Violet 610 SuperBright 600 SuperNova v605 Vio Bright V600 DyLight 405LS
		670 / 30	635	Brilliant Violet 650 Chlorophyll A StarBright Violet 670 SuperBright 645 SuperBright 702 eFluor 650NC eVolve 655 Qdot 655
		710 / 50	690	Brilliant Violet 711 Brilliant Violet 750 StarBright Violet 710 StarBright Violet 760 SuperBright 702 Qdot 705
		740 / 35	710	Brilliant Violet 711 Brilliant Violet 750 StarBright Violet 710 StarBright Violet 760 StarBright Violet 790 SuperBright 702 SuperBright 780 Qdot 705
		780 / 60	750	Aco-800 Brilliant Violet 750 Brilliant Violet 785 Brilliant Violet 786 StarBright Violet 760 StarBright Violet 790 SuperBright 780 SuperNova v786 Qdot 800

Laser	488	515 / 20	505	Brilliant Blue 515 FITC Alexa Fluor 488 CF488A CF503R Cy2 DyLight 488 Fluorescein Vio Bright FITC
		610 / 20	600	Brilliant Blue 630-P2
		670 / 30	635	Brilliant Blue 660-P2
		710 / 50	690	ATTO 490LS Brilliant Blue 700 cFluor B690 cFluor BYG680 cFluor BYG710 Di-8-ANEPPS NovaFluor Blue 690 PE-Cy5.5 PE-Dyomics 647 PE-Fire 700 PerCP-eFluor 710 PerCP-Vio700 RealBlue 705 RealBlue 744 RPE-CF647T StarBright Blue 675 StarBright Blue 700 StarBright Blue 765 SureLight PE Cyanine 5.5
		780 / 60	750	Brilliant Blue 790-P

Laser	561	575 / 25	550	AbBy Fluor 555 ABflo 555 Aco-600 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 561 ATTO 542 ATTO 550 ATTO 565 ATTO Rho3B Calcein Red AM Calcein Red-Orange AM Calcium Orange CellMask Orange Plasma Membrane Stain CF568 CF570 cFluor BYG575 cFluor YG584 CytoTrace Red DyLight 549 DyLight 550 Dyomics 547 ExoBrite 560/585 EV Membrane Stain HiLyte 555 LysoBrite Orange LysoBrite Red LysoTracker Red LysoView 550 MemBrite Fix 568/580 mFluor Blue 570 MitoLite Orange FX570 MitoLite Red FX600 MitoMark Red mScarlet NL 557 NovaFluor Yellow 570 PE PE-Dyomics 590 PhenoVue 551 Mitochondrial Stain PhenoVue 577 Lysosomal Stain PhenoVue 578 Mitochondrial Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Fluor 555 PhenoVue ROS-570, Total Oxidative Stress Indicator RayBright PE RealYellow 586 Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 Spark YG 581 XFD555
		610 / 20	600	Aco-600 ATTO 550 ATTO 565 ATTO Rho3B BOBO-3 Calcein Red-Orange AM CF568 CF570 cFluor BYG610 cFluor YG584 cFluor YG610 DyLight 549 ExoBrite 560/585 EV Membrane Stain LipidSpot 610 LysoBrite Red LysoTracker Red LysoView 550 LysoView 594 Magic Red MemBrite Fix 568/580 MitoLite Orange FX570 MitoLite Red FX600 MitoMark Red mNeptune mScarlet NovaFluor Yellow 610 PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue 577 Lysosomal Stain PhenoVue 578 Mitochondrial Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Nile Red Lipid Stain RealYellow 586 RealYellow 610 ReAsH Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 SNARF 1 pH9.0 Spark YG 581 StarBright Yellow 605 SureLight PE-594
		670 / 30	635	Aco-600 BactoView Live Red cFluor BYG667 cFluor BYG680 DRAQ 9 LipidSpot 610 LysoView 594 mNeptune NovaFluor Yellow 660 NucSpot Far-Red PE-Cy5 PE-Cy5.5 PE-Dyomics 647 PE-Fire 640 PE-Fire 700 PE-Vio670 PhenoVue Nile Red Lipid Stain PromoFluor 633P RealYellow 703 RPE-CF647T SNARF 1 pH9.0 StarBright Yellow 665 SureLight P3 SureLight PE Cyanine 5.0 SureLight PE Cyanine 5.5 SureLight RPC
		780 / 60	750	cFluor BYG781 DRAQ 9 NovaFluor Yellow 755 PE-Cy7 PE-Fire 810 PE-Vio770 RayBright PE-Cy7 RealYellow 775 Seta-R-PE-775 StarBright Yellow 800 SureLight PE Cyanine 7.0

Laser	633	670 / 30	--	AbBy Fluor 647 ABflo 647 Alexa Fluor 633 Alexa Fluor 635 Alexa Fluor 647 Alexa Fluor 660 APC ATTO 620 ATTO 633 ATTO 647 ATTO 647N ATTO 655 Calcein Deep Red AM CellTracker Deep Red CellVue Claret CF633 CF640R CF647 cFluor R685 Chromeo 642 CoraLite 647A CoraLite Plus 647 Cy5 CypHer5E DRAQ 9 DyLight 633 DyLight 649 DyLight 650 ExoBrite 640/660 EV Membrane Stain FluoroProbes647h HiLyte 647 iFluor 633 IRDye 650 IRDye 680RD Janelia Fluor 635 Janelia Fluor 646 LipidSpot 610 LysoBrite NIR LysoTracker Deep Red LysoView 640 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NL 637 NucSpot 640 NucSpot Far-Red Oyster 650 PhenoVue 641 Mitochondrial Stain PhenoVue Fluor 647 PhenoVue ROS-670, Total Oxidative Stress Indicator RayBright APC RayBright Red 647 STAR 635 STAR 635P STAR RED SureLight APC SureLight P3 SureLight RPC Thiazole Red Thiazole Red Homodimer Vio 667 Vio Bright 667 Vio Bright R667 Vio R667 XFD647
		710 / 50	690	Alexa Fluor 647 Alexa Fluor 660 APC-Alexa 680 APC-Alexa 700 APC-R700 ATTO 633 ATTO 647 ATTO 647N ATTO 655 CellTracker Deep Red CellVue Claret cFluor R685 DRAQ 9 DyLight 649 FluoroProbes647h HiLyte 647 IRDye 680RD LipidSpot 610 LysoView 640 LysoView 680 MaxLight 650 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NovaFluor Red 725 NucSpot 640 NucSpot 680 NucSpot Far-Red Oyster 650 PhenoVue Fluor 647 Red 718 Seta-APC-705 Vio 667 Vio Bright 667 Vio Bright R667 Vio Bright R720 Vio R667 Vio R720 XFD647
		780 / 60	750	APC-CF750 APC-CF770 APC-eFluor 780 APC-Fire 750 APC-Fire 810 APC-H7 APC-Vio770 DRAQ 9 LysoView 680 NovaFluor Red 755 PE-Fire 780 RayBright Red 780

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.

NYU Langone Medical Center Cytometry and Cell Sorting Core

BD FACS Symphony A5 Analyzer Configuration

355

379 / 28

--

450 / 50

410

515 / 30

450

586 / 15

550

670 / 30

635

740 / 35

690

820 / 60

770

407

450 / 50

--

474 / 25

450

586 / 15

550

610 / 20

600

670 / 30

635

710 / 50

690

740 / 35

710

780 / 60

750

488

515 / 20

505

610 / 20

600

670 / 30

635

710 / 50

690

780 / 60

750

561

575 / 25

550

610 / 20

600

670 / 30

635

780 / 60

750

633

670 / 30

--

710 / 50

690

780 / 60

750

NYU Langone Medical Center
Cytometry and Cell Sorting Core