BD FACS Aria II SORTER Configuration

	Laser (nm)	LP	Optimal Colors Additional optimal fluorochrome options available on FluoroFinder.com.
Laser	355	379 / 38	--	Aco-430 Brilliant Ultraviolet 395 mFluor UV 375 Spark UV 387 StarBright UltraViolet 400
		460 / 30	450	DAPI Hoechst (33258) DyLight 350
		730 / 45	670	Brilliant Ultraviolet 737

Laser	407	450 / 50	--	Brilliant Violet 421 Pacific Blue V450 Alexa Fluor 405 CF405M DyLight 405 eFluor 450 SuperBright 436 Vio Blue
		530 / 40	505	Aco-490 ATTO 425 ATTO 430LS Brilliant Violet 480 Brilliant Violet 510 CytoCalcein Violet 500 DY-395XL LipidSpot 488 LysoBrite Blue LysoSensor Yellow/Blue pH3.0 MitoLite Orange EX405 Spark Violet 538 Spectrum Aqua StarBright Violet 475 StarBright Violet 515 Vio Green
		585 / 20	550	ATTO 430LS Brilliant Violet 605 DY-395XL LipidSpot 488 LysoSensor Yellow/Blue pH3.0 Spark Violet 538 StarBright Violet 570 StarBright Violet 610 SuperBright 600 Vio Bright V600
		610 / 20	595	Brilliant Violet 605 Qdot 605 DyLight 405LS eFluor 605NC eVolve 605 SuperBright 600
		660 / 20	635	Brilliant Violet 650 Qdot 655 eFluor 650NC eVolve 655 SuperBright 645
		720 / 50	685	Brilliant Violet 711 Brilliant Violet 750 StarBright Violet 710 StarBright Violet 760 SuperBright 702 Qdot 705
		780 / 60	755	Brilliant Violet 785 Brilliant Violet 786 Qdot 800

Laser	488	530 / 30	505	Alexa Fluor 488 Brilliant Blue 515 CFSE FITC GFP YFP ATTO 488 CF488A CF503R Cy2
Laser	488	695 / 40	635	PE-Cy5 PerCP PerCP-Cy5.5 7-AAD Brilliant Blue 700 PerCP-eFluor 710 PerCP-Vio700

Laser	561	585 / 15	--	AbBy Fluor 555 ABflo 555 Aco-600 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 561 ATTO 542 ATTO 550 ATTO 565 ATTO Rho3B BOBO-3 Calcein Red AM Calcein Red-Orange AM Calcium Orange CellMask Orange Plasma Membrane Stain CF568 CF570 cFluor BYG575 cFluor BYG610 cFluor YG584 cFluor YG610 CytoTrace Red DyLight 549 DyLight 550 Dyomics 547 ExoBrite 560/585 EV Membrane Stain HiLyte 555 LysoBrite Orange LysoBrite Red LysoTracker Red LysoView 550 MemBrite Fix 568/580 mFluor Blue 570 MitoLite Orange FX570 MitoLite Red FX600 MitoMark Red mScarlet NL 557 NovaFluor Yellow 570 PE PE-Dazzle 594 PE-Dyomics 590 PE-eFluor 610 PhenoVue 551 Mitochondrial Stain PhenoVue 577 Lysosomal Stain PhenoVue 578 Mitochondrial Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Fluor 555 PhenoVue ROS-570, Total Oxidative Stress Indicator RayBright PE RealYellow 586 Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 Spark YG 581 StarBright Yellow 605 SureLight PE-594 XFD555
		610 / 20	600	Aco-600 ATTO 550 ATTO 565 ATTO Rho3B BOBO-3 Calcein Red-Orange AM CF568 CF570 cFluor BYG610 cFluor YG584 cFluor YG610 DyLight 549 ExoBrite 560/585 EV Membrane Stain LipidSpot 610 LysoBrite Red LysoTracker Red LysoView 550 LysoView 594 Magic Red MemBrite Fix 568/580 MitoLite Orange FX570 MitoLite Red FX600 MitoMark Red mNeptune mScarlet NovaFluor Yellow 610 PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue 577 Lysosomal Stain PhenoVue 578 Mitochondrial Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Nile Red Lipid Stain RealYellow 586 RealYellow 610 ReAsH Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 SNARF 1 pH9.0 Spark YG 581 StarBright Yellow 605 SureLight PE-594
		780 / 60	735	PE-Cy7 PE-Vio770

Laser	633	670 / 30	--	AbBy Fluor 647 ABflo 647 Alexa Fluor 633 Alexa Fluor 635 Alexa Fluor 647 Alexa Fluor 660 APC ATTO 620 ATTO 633 ATTO 647 ATTO 647N ATTO 655 Calcein Deep Red AM CellTracker Deep Red CellVue Claret CF633 CF640R CF647 cFluor R685 Chromeo 642 CoraLite 647A CoraLite Plus 647 Cy5 CypHer5E DRAQ 9 DyLight 633 DyLight 649 DyLight 650 ExoBrite 640/660 EV Membrane Stain FluoroProbes647h HiLyte 647 iFluor 633 IRDye 650 IRDye 680RD Janelia Fluor 635 Janelia Fluor 646 LipidSpot 610 LysoBrite NIR LysoTracker Deep Red LysoView 640 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NL 637 NucSpot 640 NucSpot Far-Red Oyster 650 PhenoVue 641 Mitochondrial Stain PhenoVue Fluor 647 PhenoVue ROS-670, Total Oxidative Stress Indicator RayBright APC RayBright Red 647 STAR 635 STAR 635P STAR RED SureLight APC SureLight P3 SureLight RPC Thiazole Red Thiazole Red Homodimer Vio 667 Vio Bright 667 Vio Bright R667 Vio R667 XFD647
		710 / 50	690	Alexa Fluor 647 Alexa Fluor 660 APC-Alexa 680 APC-Alexa 700 APC-R700 ATTO 633 ATTO 647 ATTO 647N ATTO 655 CellTracker Deep Red CellVue Claret cFluor R685 DRAQ 9 DyLight 649 FluoroProbes647h HiLyte 647 IRDye 680RD LipidSpot 610 LysoView 640 LysoView 680 MaxLight 650 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NovaFluor Red 725 NucSpot 640 NucSpot 680 NucSpot Far-Red Oyster 650 PhenoVue Fluor 647 Red 718 Seta-APC-705 Vio 667 Vio Bright 667 Vio Bright R667 Vio Bright R720 Vio R667 Vio R720 XFD647
		780 / 60	755	Alexa Fluor 750 APC-Cy7 APC-H7 APC-Alexa 750 APC-eFluor 780 APC-Fire 750 APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.

NYU Langone Medical Center Cytometry and Cell Sorting Core

BD FACS Aria II SORTER Configuration

355

379 / 38

--

460 / 30

450

730 / 45

670

407

450 / 50

--

530 / 40

505

585 / 20

550

610 / 20

595

660 / 20

635

720 / 50

685

780 / 60

755

488

530 / 30

505

695 / 40

635

561

585 / 15

--

610 / 20

600

780 / 60

735

633

670 / 30

--

710 / 50

690

780 / 60

755

NYU Langone Medical Center
Cytometry and Cell Sorting Core