BD FACSMelody Configuration

	Laser (nm)	LP	Optimal Colors Additional optimal fluorochrome options available on FluoroFinder.com.
Laser	488	527 / 32	507	AbBy Fluor 488 ABflo 488 Adirondack Green 520 Alexa Fluor 488 Alpha Fluor 488 ATTO 488 ATTO 495 BactoView Dead 500/515 BactoView Live Green BCECF AM BDY Lenalidomide Brilliant Blue 515 CellTrace Oregon Green CellTracker Green CF488A CF500 cFluor B515 cFluor B532 cFluor B548 Chromeo 488 Chromeo 505 CoraLite 532 CoraLite Plus 488 CytoTrace Green CMFDA DAF-FM DMAO DyLight 488 ExoBrite 490/515 EV Membrane Stain FITC FITC Plus HiLyte 488 iFluor 488 KIRAVIA Blue 520 LysoBrite Green LysoView 488 MaxLight 490 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoMark Green MitoROS 520 Mpro Tracer NBD Neuro DiO NL 493 NovaFluor Blue 530 Nuclear Green Nuclear Orange NucSpot 470 NucSpot Live 488 Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator PicoGreen PromoFluor 488P RayBright Blue 488 RealBlue 545 Spark Blue 574 Spectrum Green SureLight 488 Syto 16 Syto 24 Thiazole Orange Homodimer Thiazole Orange Iodide Vio 488 Vio 515 Vio Bright 515 Vio Bright FITC VRDye 490 XFD488
Laser	488	670 / 30	640	Aco-600 ATTO 490LS Brilliant Blue 700 cFluor B690 cFluor BYG667 cFluor BYG680 Chromeo 494 Di-8-ANEPPS NovaFluor Blue 660 / 40S NovaFluor Blue 690 PE-Cy5 PE-Cy5.5 PE-Dyomics 647 PE-Fire 640 PE-Fire 700 PerCP-eFluor 710 PerCP-Vio700 PE-Vio670 QM-FN-SO3 RMR-Tre RPE-CF647T StarBright Blue 675 StarBright Blue 700 SureLight PE Cyanine 5.0 SureLight PE Cyanine 5.5

Laser	405	448 / 45	--	AbBy Fluor 405 ABflo 405 ABflo 450 Aco-490 Alexa Fluor 405 Brilliant Violet 421 Brilliant Violet 480 CF405M CF405S cFluor V420 cFluor V450 CoraLite Plus 405 CytoCalcein Violet 450 CytoCalcein Violet 500 DY-415 DyLight 405 eFluor 450 ExoBrite 410/450 EV Membrane Stain HiLyte 405 iFluor 405 LysoBrite Blue LysoView 405 MaxLight 405 MemBrite Fix 405/430 mFluor Violet 450 MitoLite Blue FX490 MitoView 405 Nuclear Violet Nuclear Yellow Oxazole Blue Homodimer Pacific Blue POPO-1 PromoFluor 405 RayBright Violet 450 Spark Violet 423 Spectrum Aqua StarBright Violet 440 StarBright Violet 475 SuperBright 436 SuperNova v428 Tricyclic cytosine tC V450 Vio Blue Vio Bright V423 violetFluor 450
Laser	405	528 / 45	500	Aco-490 ATTO 430LS Brilliant Violet 480 Brilliant Violet 510 CytoCalcein Violet 500 DY-395XL LipidSpot 488 LysoBrite Blue LysoSensor Yellow/Blue pH3.0 MitoLite Blue FX490 MitoLite Orange EX405 Nuclear Yellow Spark Violet 538 Spectrum Aqua StarBright Blue 520 StarBright Violet 475 StarBright Violet 515 Tricyclic cytosine tC Vio Green

Laser	561	582 / 15	582	AbBy Fluor 555 ABflo 555 Aco-600 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 561 ATTO 550 ATTO 565 ATTO Rho3B BOBO-3 BOP-JF549 Calcein Red AM Calcein Red-Orange AM Calcium Orange CellMask Orange Plasma Membrane Stain CF568 CF570 cFluor BYG575 cFluor YG584 CytoTrace Red DyLight 549 Dyomics 547 ExoBrite 560/585 EV Membrane Stain HiLyte 555 LysoBrite Orange LysoBrite Red LysoTracker Red LysoView 550 MemBrite Fix 568/580 mFluor Blue 570 MitoLite Orange FX570 MitoLite Red FX600 MitoMark Red mScarlet NL 557 NovaFluor Yellow 570 PE-Dyomics 590 PhenoVue 551 Mitochondrial Stain PhenoVue 577 Lysosomal Stain PhenoVue 578 Mitochondrial Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Fluor 555 PhenoVue ROS-570, Total Oxidative Stress Indicator RayBright PE RealYellow 586 Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 Spark YG 581 StarBright Yellow 605 Taxol Janelia Fluor 549 XFD555
		613 / 18	605	Aco-600 ATTO 565 ATTO Rho3B BOBO-3 Calcein Red-Orange AM CF570 cFluor BYG610 cFluor YG610 LipidSpot 610 LysoBrite Red LysoTracker Red LysoView 550 LysoView 594 Magic Red MitoLite Red FX600 MitoMark Red mNeptune mScarlet NovaFluor Yellow 610 PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue 577 Lysosomal Stain PhenoVue 578 Mitochondrial Stain PhenoVue Nile Red Lipid Stain RealYellow 586 RealYellow 610 ReAsH Rhodamine Red SNARF 1 pH6.0 SNARF 1 pH9.0 StarBright Yellow 605 SureLight PE-594 SureLight RPC
		697 / 58	655	BactoView Live Red cFluor BYG667 cFluor BYG680 cFluor BYG710 DRAQ 9 LipidSpot 610 LysoView 594 mNeptune NovaFluor Yellow 660 NucSpot Far-Red PE-Cy5 PE-Cy5.5 PE-Dyomics 647 PE-Fire 700 PE-Vio670 PhenoVue Nile Red Lipid Stain PromoFluor 633P QM-FN-SO3 RealYellow 703 RMR-Tre RPE-CF647T SNARF 1 pH9.0 StarBright Yellow 665 StarBright Yellow 720 SureLight P3 SureLight PE Cyanine 5.0 SureLight PE Cyanine 5.5
		783 / 56	752	cFluor BYG781 DRAQ 9 NovaFluor Yellow 755 PE-Cy7 PE-Fire 810 PE-Vio770 QM-FN-SO3 RayBright PE-Cy7 RealYellow 775 Seta-R-PE-775 StarBright Yellow 800 SureLight PE Cyanine 7.0

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.

University of Manitoba University of Manitoba Flow Core

BD FACSMelody Configuration

488

527 / 32

507

670 / 30

640

405

448 / 45

--

528 / 45

500

561

582 / 15

582

613 / 18

605

697 / 58

655

783 / 56

752

University of Manitoba
University of Manitoba Flow Core