Koch Institute at MIT
The Swanson Biotechnology Center Flow Cytometry Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/mit-swanson
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FACS Aria 2 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 40

--

 Brilliant Violet 421
DAPI
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

--

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

610 / 20

--

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

660 / 20

--

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

--

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

--

 Alexa Fluor 488
Brilliant Blue 515
GFP
ATTO 488
CF488A
CF503R
Cy2
DyLight 488
FITC
Fluorescein

695 / 40

--

 Brilliant Blue 700
PerCP-Cy5.5
Laser

561

582 / 15

--

 dTomato
PE
CF550R
CF568
CF570

610 / 20

--

 mCherry
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 14

--

 PE-Cy5.5

780 / 60

--

 PE-Cy7
PE-Vio770
Laser

640

660 / 20

--

 Alexa Fluor 647
APC
Cy5
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

780 / 60

--

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.