Koch Institute at MIT
The Swanson Biotechnology Center Flow Cytometry Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/mit-swanson
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LSR Fortessa HTS-2 (355/405/488/640) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

376 / 28

--

 Brilliant Ultraviolet 395

515 / 30

--

 Brilliant Ultraviolet 496

670 / 30

--

 Brilliant Ultraviolet 661

740 / 35

--

 Brilliant Ultraviolet 737

820 / 60

--

 Brilliant Ultraviolet 805
Laser

405

450 / 50

--

 Brilliant Violet 421
DAPI
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

--

 AmCyan
Brilliant Violet 510
Live/Dead Fix Aqua
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

610 / 20

--

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

710 / 50

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

--

 Brilliant Violet 785
Qdot 800
Laser

488

530 / 30

--

 Alexa 488 NanoGold
Alexa Fluor 488
Alexa Fluor 532
ATTO 488
Brilliant Blue 515
CYTO-ID Green
DyLight 488
eYFP
FITC
GFP
Vio Bright FITC
Vybrant DyeCycle Green

575 / 25

--

 Alexa Fluor 532
Cy3
CYTO-ID Green
CyTRAK Orange
PE
Vybrant DyeCycle Green
Alexa Fluor 555
Cyanine 3 SE

610 / 20

--

 PE-Texas Red
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

710 / 50

--

780 / 60

--

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

730 / 45

--

 Alexa Fluor 700
APC-Cy5.5
APC-Cy7
APC-R700
ATTO 655
CellVue Plum
Vybrant DyeCycle Ruby
Alexa Fluor 680
DyLight 680

780 / 60

--

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.