MD Anderson Cancer Center
Advanced Cytometry and Sorting Facility

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LSRFortessa Analyzer Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 DAPI
Hoechst (33258)
Live/Dead Fix Blue
DyLight 350

730 / 45

--

 Brilliant Ultraviolet 737
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
Calcein Blue AM
eFluor 450
Pacific Blue
Sytox Blue
CF405M
DyLight 405
SuperBright 436
V450

525 / 50

--

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
CFP
Live/Dead Fix Aqua
CF405L
CF430
CF440
DyLight 405LS
Krome Orange

605 / 12

--

 Brilliant Violet 605
Pacific Orange
Qdot 605
Alexa Fluor 430
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

670 / 30

--

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

695 / 40

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

--

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

530 / 30

--

 Alexa Fluor 488
Calcein AM
FITC
GFP
Sytox Green
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

710 / 50

--

 PerCP
PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 15

--

 Alexa Fluor 555
dsRed
PE
Propidium Iodide
CF550R
CF568
CF570
Cy3

610 / 20

--

 Alexa Fluor 610
mCherry
mKate2
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594

660 / 20

--

 PE-Cy5
PE-Dyomics 647

710 / 50

--

 PE-Alexa 700
PE-Cy5.5

780 / 60

--

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

640

670 / 14

--

 Alexa Fluor 647
APC
Sytox Red
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

730 / 45

--

 Alexa Fluor 700
DyLight 680
APC-R700

780 / 60

--

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.