SickKids - UHN
Flow Cytometry Facility-PGCRL node

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LSR Fortessa (BGRVU) PGCRL (Room 18-9430) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

515 / 30

--

 Brilliant Ultraviolet 496
DAPI
eFluor 455UV Fix Viability
FVS440UV
Hoechst (33258)
Hoechst (33342)
Live/Dead Fix Blue
Zombie UV

740 / 35

685

 Brilliant Ultraviolet 737
Laser

405

431 / 28

--

 Alexa Fluor 405
Brilliant Violet 421
eBFP
eFluor 450
eFluor 450 Fix Viability
FVS450
Live/Dead Fix Violet
Pacific Blue
SuperBright 436
V450
Zombie Violet

525 / 50

505

 Alexa Fluor 430
AmCyan
Brilliant Violet 480
Brilliant Violet 510
eCFP
eFluor 506
eFluor 506 Fix Viability
FVS510
Live/Dead Fix Aqua
Pacific Orange
Qdot 525
V500
Zombie Aqua

610 / 20

600

 Brilliant Violet 605
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
DyLight 405LS

660 / 20

630

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
SuperBright 780
Laser

488

515 / 30

--

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
FVS520
GFP
Zombie Green
ATTO 488
CF488A
CF503R

710 / 50

685

 Brilliant Blue 700
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
PE-Cy5
PE-Cy5.5
Laser

532

575 / 26

--

 Alexa Fluor 546
Alexa Fluor 555
CellROX Orange
Cy3
dsRed
eFluor 570
FVS570
PE
RFP
Sytox Orange
tdTomato

610 / 20

600

 FVS620
Live/Dead Fix Red
mCherry
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Texas Red
Propidium Iodide
Sytox AADvanced
7-AAD

780 / 60

750

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 647
APC
CellROX Deep Red
Cy5
eFluor 660
eFluor 660 Fix Viability
FVS660
Live/Dead Fix Far Red
Sytox Red

720 / 40

685

 Alexa Fluor 700
APC-R700
FVS700

780 / 60

755

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770
eFluor 780 Fix Viability
FVS780
Live/Dead Fix Near IR (775)
Zombie NIR

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.