Ludwig Maximilian University of Munich (LMU)
BMC - CFFlowCyt

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ImageStream (NC 03.044) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

457 / 45

--

 Brilliant Violet 421
DAPI
Pacific Blue
SuperBright 436
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

537 / 65

--

 Alexa Fluor 430
Brilliant Violet 510
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
Pacific Orange
V500
Vio Green

610 / 30

--

 Brilliant Violet 605
eFluor 625NC
Qdot 625
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605

702 / 85

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

762 / 35

--

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

528 / 65

--

 Alexa Fluor 488
CFSE
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

577 / 35

--

 Cy3
PE
Alexa Fluor 555
Cyanine 3 SE

610 / 30

--

 ECD
PE-Alexa 610
PE-Texas Red
Propidium Iodide
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

702 / 85

--

 DRAQ 5
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

762 / 35

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

561

577 / 35

--

 Alexa Fluor 546
Cy3
PE
Alexa Fluor 555
ATTO 550
CF550R
CF568
CF570
Cyanine 3 SE
DyLight 549

610 / 30

--

 Alexa Fluor 568
ECD
mCherry
PE-Texas Red
RFP
CF583
CF583R
Cy3.5
DyLight 594
Dyomics 590

702 / 85

--

 PE-Alexa 647
PE-Cy5
7-AAD

762 / 35

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

592

610 / 30

--

 Alexa Fluor 594
mCherry
Texas Red
ATTO 594

702 / 85

--

 Alexa Fluor 647
Alexa Fluor 660
APC
Cy5
DRAQ 5

762 / 35

--

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
Laser

642

702 / 85

--

 Alexa Fluor 647
Alexa Fluor 660
APC
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

762 / 35

--

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.