Ludwig Maximilian University of Munich (LMU)
BMC - CFFlowCyt

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BD Fusion No1 (NB 00.049) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

DAPI
DyLight 350

530 / 30

505

Brilliant Ultraviolet 496
Laser

405

450 / 40

--

Brilliant Violet 421
Pacific Blue
SuperBright 436
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

525 / 50

505

AmCyan
Brilliant Violet 510
Krome Orange
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

610 / 20

595

Brilliant Violet 605
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605

660 / 20

630

Brilliant Violet 650
SuperBright 645
eFluor 650NC
eVolve 655
Qdot 655

710 / 50

690

Brilliant Violet 711
SuperBright 702
Qdot 705

780 / 60

750

Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

502

Alexa Fluor 488
CFSE
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

695 / 40

655

PerCP
PerCP-Cy5.5
Propidium Iodide
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

586 / 15

570

dsRed
dTomato
PE
CF550R
CF568
CF570

610 / 20

600

mCherry
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 14

630

PE-Cy5

710 / 50

685

PE-Cy5.5

780 / 60

735

PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

640

670 / 30

--

Alexa Fluor 647
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
APC
CF640R
CF647
Cy5
DyLight 650
Dyomics 647

730 / 45

690

Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

750

APC-Cy7
APC-eFluor 780
APC-Alexa 750
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.