Medical Center - University of Freiburg
Lighthouse Core Facility

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LSR Fortessa Andy (405, 488, 640) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
ATTO 425
Brilliant Violet 421
Brilliant Violet 480
Calcein Violet AM
CellTrace Violet
DY-415
DyLight 405
eFluor 450
Pacific Blue
V450
Vio Blue
Vybrant DyeCycle Violet

525 / 50

502

 Alexa Fluor 430
ATTO 425
Brilliant Violet 480
Brilliant Violet 510
Calcein Violet AM
CellTrace Violet
V500
Vio Green
AmCyan
Cascade Yellow

610 / 20

595

 Brilliant Violet 605
eFluor 605NC
DyLight 405LS
eVolve 605
Qdot 605
SuperBright 600

660 / 20

630

 Brilliant Violet 650
Fura Red AM (Ca2+bound)
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
GFP
YFP
ATTO 488
CF488A
CF503R
Cy2

575 / 26

550

 dsRed
PE
RFP
tdTomato

610 / 20

600

 PE-Texas Red
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

710 / 50

685

 Brilliant Blue 700
PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
7-AAD

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 14

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 Alexa Fluor 633
Alexa Fluor 647
APC
DRAQ 5
eFluor 660
TO-Pro 3
Alexa Fluor 635
CF640R
CF647
Cy5

730 / 45

710

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Cy7
APC-eFluor 780
APC-H7
eFluor 780 Fix Viability
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.