Centre de Recherche du CHU de Quebec-Universite Laval
Plateforme de Cytometrie

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LSRII - Disabled 17JAN24 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

DAPI
Hoechst (33258)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
DyLight 350

525 / 50

505

Indo-Lo (Ca2+free)
Laser

405

450 / 50

--

Pacific Blue
V450
Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
Vio Blue

525 / 50

505

AmCyan
eFluor 506
Lucifer Yellow
V500
Alexa Fluor 430
Brilliant Violet 510
DyLight 405LS
Vio Green

610 / 20

600

Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
Laser

488

525 / 25

505

Alexa Fluor 488
CFSE
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

670 / 14

635

PerCP
Laser

531

585 / 15

--

Alexa Fluor 546
PE
Alexa Fluor 555
CF550R
CF570
Cy3
Dyomics 547
eFluor 570
Phalloidin-eFluor 570
TMRE

610 / 20

600

Cy3
mCherry
PE-Texas Red
Cyanine 3 SE
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

630

PE-Cy5

780 / 60

735

PE-Cy7
PE-Vio770
Laser

640

660 / 20

--

Alexa Fluor 633
Alexa Fluor 647
APC
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

780 / 60

735

APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.