Thomas Jefferson University
Sidney Kimmel Cancer Center Flow Cytometry Shared Resource

Design a panel and find antibodies today at

https://app.fluorofinder.com/jefferson
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LSR II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 DAPI
Pacific Blue
V450
Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
Vio Blue

525 / 50

505

 AmCyan
Qdot 525
Alexa Fluor 430
Brilliant Violet 510
V500
Vio Green

585 / 42

570

 Pacific Orange
Qdot 585
Alexa Fluor 430
Brilliant Violet 570
DyLight 405LS
Krome Orange
Laser

488

530 / 30

505

 Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

670 / 14

635

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
Laser

543

582 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 PE-Texas Red
RFP
CF583
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

695 / 40

685

 PE-Cy5
PE-Cy5.5

780 / 60

735

 PE-Cy7
Laser

633

670 / 30

--

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

720 / 40

690

 Alexa Fluor 700
APC-R700

780 / 60

735

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.