Agency for Science, Technology and Research (A*STAR)
IMCB Flow Cytometry Core Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/imcb-flowcore
Logo: FluoroFinder

BD LSRII Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

Brilliant Ultraviolet 395

450 / 50

--

DAPI
DyLight 350

730 / 45

--

Brilliant Ultraviolet 737
Laser

405

450 / 50

--

Brilliant Violet 421
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

505

Brilliant Violet 510
Pacific Orange
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
V500
Vio Green

610 / 20

--

Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

660 / 20

--

Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

--

Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

--

Alexa Fluor 488
FITC
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
NL 493

575 / 26

--

Alexa Fluor 514
Alexa Fluor 532
PE-Cy7

695 / 40

--

PerCP

780 / 60

--

PE-Cy7
PE-Vio770
Laser

640

660 / 20

--

Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

780 / 60

--

APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.