Agency for Science, Technology and Research (A*STAR)
IMCB Flow Cytometry Core Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/imcb-flowcore

BD FACSSymphony Analyzer Configuration

	Laser (nm)	SP	LP	Optimal Colors Additional optimal fluorochrome options available on FluoroFinder.com.
Laser	355	379 / 34	--	370	Brilliant Ultraviolet 395
		442 / 43	--	--	DAPI FVS440UV Hoechst (33258) DyLight 350
		465 / 29	--	455	Brilliant Ultraviolet 496
		510 / 40	--	495	Brilliant Ultraviolet 496
		530 / 50	--	530	Brilliant Ultraviolet 496
		585 / 30	--	--	Brilliant Ultraviolet 563
		615 / 25	--	605	Brilliant Ultraviolet 615-P
		670 / 50	--	650	Brilliant Ultraviolet 661
		730 / 50	--	--	Brilliant Ultraviolet 737
		750 / 30	--	740	Brilliant Ultraviolet 737
		780 / 60	--	755	Brilliant Ultraviolet 737
		800 / 60	--	785	Brilliant Ultraviolet 805

Laser	405	442 / 43	--	--	Brilliant Violet 421 FVS450 Pacific Blue Alexa Fluor 405 CF405M DyLight 405 eFluor 450 SuperBright 436 V450 Vio Blue
		465 / 29	--	455	Brilliant Violet 480
		510 / 30	--	--	AmCyan Brilliant Violet 510 FVS510 Alexa Fluor 430 CF405L CF430 CF440 V500 Vio Green
		530 / 50	--	525	Pacific Orange Alexa Fluor 430 Brilliant Violet 510 Brilliant Violet 570 DyLight 405LS Krome Orange V500 Vio Green
		585 / 30	--	--	Brilliant Violet 570 Pacific Orange Alexa Fluor 430 DyLight 405LS Krome Orange Qdot 565 Qdot 585
		595 / 33	--	590	Brilliant Violet 570 DyLight 405LS Qdot 585
		615 / 25	--	605	Brilliant Violet 605 DyLight 405LS eFluor 605NC eVolve 605 Qdot 605 SuperBright 600
		670 / 50	--	650	Brilliant Violet 650 eFluor 650NC eVolve 655 Qdot 655 SuperBright 645
		710 / 50	--	690	Brilliant Violet 711 Qdot 705 SuperBright 702
		750 / 30	--	740	Brilliant Violet 750
		780 / 60	--	755	Brilliant Violet 785 Brilliant Violet 786 Qdot 800
		800 / 60	--	785	Brilliant Violet 786 Qdot 800

Laser	488	510 / 30	--	--	Brilliant Blue 515 Alexa Fluor 488 CF488A CF503R DyLight 488 FITC Vio Bright FITC
		530 / 50	--	525	Brilliant Blue 515 eGFP FITC FVS520 GFP Sytox Green Alexa Fluor 488 CF488A CF503R Cy2
		585 / 30	--	--	PE
		610 / 30	--	--	Brilliant Blue 630-P2
		660 / 30	--	650	PE-Alexa 647 PE-Cy5 PerCP-Vio700
		680 / 30	--	670	Brilliant Blue 700 PerCP-Cy5.5
		710 / 50	--	690	Brilliant Blue 700 PerCP-Cy5.5
		770 / 40	950	--	Brilliant Blue 790-P
		800 / 60	--	785	PE-Cy7 PE-Vio770

Laser	532	580 / 20	--	--	mOrange PE RFP CF550R CF570
		610 / 30	--	--	Alexa Fluor 568 Alexa Fluor 594 mCherry PE-CF594 PE-Texas Red BODIPY TR ECD PE-Dazzle 594 PE-eFluor 610 PE-Vio615
		660 / 30	--	--	PE-Alexa 647 PE-Cy5
		680 / 30	--	--	PE-Cy5.5
		700 / 40	--	--	PE-Cy5
		725 / 20	--	695	PE-Alexa 700
		750 / 40	--	--	PE-Alexa 700 PE-Vio770 PE-Alexa 750 PE-Cy7
		780 / 60	--	--	PE-Cy7 PE-Vio770
		800 / 60	--	800	PE-Alexa 750

Laser	628	660 / 30	--	650	Alexa Fluor 633 APC Alexa Fluor 647 CF640R Cy5 DyLight 633 NL 637
		680 / 30	--	670	Alexa Fluor 660
		710 / 50	--	695	Alexa Fluor 680 Alexa Fluor 700 APC-R700 FVS700 APC-Cy5.5 DyLight 680
		750 / 60	--	740	Alexa Fluor 700 APC-Alexa 700 APC-H7 APC-Alexa 750 APC-Cy7 APC-eFluor 780 APC-Fire 750 APC-R700 APC-Vio770
		780 / 60	--	760	APC-Cy7 APC-Alexa 750 APC-eFluor 780 APC-Fire 750 APC-H7 APC-Vio770
		820 / 30	--	--	APC-CF750 APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.

Agency for Science, Technology and Research (A*STAR) IMCB Flow Cytometry Core Facility

BD FACSSymphony Analyzer Configuration

355

379 / 34

--

370

442 / 43

--

--

465 / 29

--

455

510 / 40

--

495

530 / 50

--

530

585 / 30

--

--

615 / 25

--

605

670 / 50

--

650

730 / 50

--

--

750 / 30

--

740

780 / 60

--

755

800 / 60

--

785

405