Institute of Cancer Research
ICR Flow Cytometry Facility

Design a panel and find antibodies today at

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Chelsea LSRII Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

DAPI

525 / 50

505

Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

660 / 20

635

Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645
Laser

488

530 / 30

505

Alexa Fluor 488
FITC
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
NL 493

575 / 25

556

PE

610 / 20

600

PE-Texas Red
Propidium Iodide
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

660 / 20

635

PE-Cy5
PerCP-Vio700

695 / 40

685

PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

735

PE-Cy7
PE-Vio770
Laser

561

610 / 20

550

PE-CF594
DyLight 594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615

695 / 40

600

dsRed
Laser

640

660 / 20

--

Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

730 / 45

685

Alexa Fluor 700
APC-R700

780 / 60

755

APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.