Houston Methodist Hospital
Houston Methodist Hospital Research Institute - Houston Methodist Flow Cytometry Core

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FACS Symphony A5SE Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

349

379 / 34

365

 Brilliant Ultraviolet 395

446 / 67

425

515 / 60

495

 Brilliant Ultraviolet 496

540 / 20

535

585 / 30

570

 Brilliant Ultraviolet 563

610 / 30

595

660 / 30

645

 Brilliant Ultraviolet 661

695 / 40

675

736 / 64

704

 Brilliant Ultraviolet 737

809 / 82

765

 Brilliant Ultraviolet 805
Laser

405

427 / 25

415

 Brilliant Violet 421
Alexa Fluor 405
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

450 / 40

430

470 / 15

465

 Brilliant Violet 480

510 / 40

495

540 / 20

530

576 / 20

570

595 / 30

585

615 / 25

605

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

660 / 30

645

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

680 / 30

665

710 / 40

690

 Brilliant Violet 711
Qdot 705
SuperBright 702

750 / 40

730

785 / 50

765

 Brilliant Violet 786
Qdot 800

845 / 70

810
Laser

488

510 / 20

500

 Brilliant Blue 515
Alexa Fluor 488
CF488A
CF503R
DyLight 488
FITC
Vio Bright FITC

537 / 32

520

576 / 20

570

602 / 40

585

660 / 30

645

675 / 20

665

710 / 50

685

 Brilliant Blue 700
PerCP-Cy5.5

750 / 60

724

810 / 79

770

 Brilliant Blue 790-P
Laser

561

585 / 30

570

 PE
CF550R
CF568
CF570

602 / 40

595

 PE-CF594
DyLight 594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615

660 / 30

645

 PE-Cy5

670 / 20

665

695 / 40

680

730 / 50

699

750 / 40

735

780 / 60

750

 PE-Cy7
PE-Vio770

825 / 49

800
Laser

637

660 / 30

645

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

675 / 20

665

680 / 30

680

710 / 25

699

 Alexa Fluor 700
APC-R700

730 / 50

720

780 / 60

750

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.