Houston Methodist Hospital
Houston Methodist Hospital Research Institute - Houston Methodist Flow Cytometry Core

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Fortessa Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

--

 DAPI
DyLight 350

530 / 30

--

505

 Hoechst (33342)
Laser

405

450 / 50

--

--

 Brilliant Violet 421
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

--

505

 AmCyan
Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

610 / 20

--

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

710 / 50

--

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

--

750

 Brilliant Violet 786
Qdot 800
Laser

488

525 / 50

--

505

 FITC
GFP
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

685 / 35

--

670

 PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 15

--

550

 Alexa Fluor 555
PE
CF550R
CF568
CF570
Cy3

610 / 20

--

600

 PE-Texas Red
Propidium Iodide
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

--

635

 PE-Cy5

710 / 50

--

685

 PE-Cy5.5

780 / 60

--

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 14

--

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

710 / 50

685

--

 Alexa Fluor 680
APC-Cy5.5
DyLight 680

780 / 60

--

750

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.