Houston Methodist Hospital
Houston Methodist Hospital Research Institute - Houston Methodist Flow Cytometry Core

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Aria II-NIR (6 lasers) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 DAPI
Hoechst (33258)
DyLight 350

530 / 30

505

 Indo-Lo (Ca2+free)
Laser

405

450 / 40

--

 Brilliant Violet 421
Pacific Blue
Sytox Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

505

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

670 / 30

630

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

705

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 FITC
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

710 / 50

685

 PerCP
Laser

551

582 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 PE-Texas Red
CF583
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

635

 PE-Cy5

710 / 50

685

 PE-Cy5.5

780 / 60

750

 PE-Cy7
Laser

640

670 / 30

--

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

710 / 50

690

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770
Laser

785

800 / 30

--

 FVS780

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.