Johns Hopkins University
Kimmel Cancer Center Flow Cytometry Shared Resource

Design a panel and find antibodies today at

https://app.fluorofinder.com/hopkins-kimmel
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Aria II BSL-3 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

Brilliant Violet 421
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

530 / 30

--

AmCyan
Brilliant Violet 510
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

605 / 40

--

Brilliant Violet 570
Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 585
Qdot 605
SuperBright 600
Laser

488

530 / 30

--

Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

585 / 42

--

PE

660 / 20

--

PE-Cy5
PerCP
PerCP-Vio700

710 / 50

--

PE-Cy5.5
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

--

PE-Cy7
PE-Vio770
Laser

640

660 / 20

--

Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

730 / 45

--

Alexa Fluor 700
APC-R700

780 / 60

--

APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.