University of Helsinki
Flow Cytometry Unit

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Biomedicum- Influx Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

460 / 50

420

 Alexa Fluor 405
Brilliant Violet 421
DAPI
eBFP
eFluor 450
Pacific Blue
V450
CF405M
DyLight 405
Vio Blue

520 / 35

480

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
Pacific Green
Pacific Orange
V500
CF405L
CF430
CF440
DY-380XL

610 / 20

550

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 20

645

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

--

750

 Brilliant Violet 750
Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

530 / 40

505

 Alexa Fluor 488
Brilliant Blue 515
eGFP
FITC
ATTO 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

670 / 30

600

 PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 29

570

 Alexa Fluor 561
dsRed
mOrange
PE
RFP
tdTomato
CF550R
CF568
CF570

615 / 20

600

 mCherry
PE-Alexa 610
PE-CF594
PE-eFluor 610
PE-Texas Red
Propidium Iodide
CF583
CF583R
DyLight 594
ECD

670 / 30

645

 mPlum
PE-Cy5
Propidium Iodide
7-AAD

710 / 50

685

 PE-Cy5.5

--

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

650

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

720 / 40

700

 Alexa Fluor 700
APC-R700

--

750

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770
Laser

785

725 / 40

700

--

835
Laser

685

725 / 40

700

--

835

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.