University of Helsinki
Flow Cytometry Unit

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Biomedicum- FACSAria II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 40

--

 Alexa Fluor 405
Brilliant Violet 421
DAPI
eBFP
eFluor 450
Pacific Blue
V450
CF405M
DyLight 405
SuperBright 436

530 / 30

502

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
Pacific Green
Pacific Orange
V500
CF405L
CF430
CF440
DY-380XL
Laser

488

530 / 30

502

 Alexa Fluor 488
Brilliant Blue 515
eGFP
FITC
ATTO 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

576 / 26

556

 PE

610 / 20

595

 PE-CF594
PE-Texas Red
Propidium Iodide
ECD
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

695 / 40

655

 PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

720 / 45

685

 Alexa Fluor 700
APC-R700

780 / 60

735

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.