George Washington University (GW)
GW Flow Cytometry Core Facility

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BD Influx Cell Sorter (405/488/561/633) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

425 / 26

--

 Alexa Fluor 405
Brilliant Violet 421
Calcein Violet AM
DAPI
eFluor 450
Zombie Violet
DyLight 405
SuperBright 436
V450
Vio Blue

520 / 35

--

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
CFP
Live/Dead Fix Aqua
Live/Dead Fix Yellow
Qdot 525
Zombie Aqua
CF405L
CF430

610 / 20

--

 Brilliant Violet 605
Pacific Orange
Qdot 605
Zombie Yellow
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 20

--

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

710 / 50

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

--

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

530 / 40

--

 Alexa Fluor 488
Calcein AM
CFSE
FITC
GFP
Sytox Green
Vybrant DyeCycle Green
YFP
Zombie Green
ATTO 488

692 / 40

--

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700
Laser

561

585 / 29

--

 Alexa Fluor 555
dsRed
PE
Sytox Orange
tdTomato
CF550R
CF568
CF570
Cy3

610 / 20

--

 Alexa Fluor 594
ECD
mCherry
mKate2
PE-Texas Red
Propidium Iodide
Zombie Red
BODIPY TR
CF583
CF583R

670 / 30

--

 PE-Cy5
7-AAD

--

750

 PE-Cy7
PE-Vio770
Laser

633

670 / 30

--

 Alexa Fluor 647
APC
E2-Crimson
Sytox Red
Vybrant DyeCycle Ruby
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5

720 / 40

--

 Alexa Fluor 700
APC-Cy5.5
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

--

 APC-Cy7
APC-eFluor 780
APC-H7
Zombie NIR
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.