University of California, San Francisco (UCSF)
Gladstone Flow Cytometry Core

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Imagestream Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

--

505

430

Alexa Fluor 405
ATTO 425
Brilliant Violet 421
Brilliant Violet 480
Brilliant Violet 510
CF405M
CF405S
cFluor V420
cFluor V450
CoraLite Plus 405
CytoCalcein Violet 450
CytoCalcein Violet 500
DY-415
DyLight 405
eFluor 450
ExoBrite 410/450 EV Membrane Stain
HiLyte 405
iFluor 405
LysoBrite Blue
LysoSensor Yellow/Blue pH3.0
LysoView 405
MaxLight 405
MemBrite Fix 405/430
mFluor Violet 450
MitoLite Blue FX490
MitoLite Orange EX405
MitoView 405
Nuclear Violet
Nuclear Yellow
Oxazole Blue Homodimer
Pacific Blue
POPO-1
PromoFluor 405
Spark Violet 423
Spark Violet 538
Spectrum Aqua
StarBright Violet 440
StarBright Violet 475
SuperBright 436
SuperNova v428
V450
Vio Blue
Vio Bright V423
Vio Green
violetFluor 450
Laser

488

--

560

505

Adirondack Green 520
Alexa Fluor 488
Alexa Fluor 555
Alpha Fluor 488
ATTO 488
BactoView Live Green
BCECF AM
Brilliant Blue 515
CellTrace Oregon Green
CF488A
CF500
cFluor B515
cFluor B532
cFluor B548
Chromeo 488
Chromeo 505
CoraLite 532
CoraLite Plus 488
CytoTrace Green CMFDA
DAF-FM
DMAO
DyLight 488
ExoBrite 490/515 EV Membrane Stain
FITC
FITC Plus
HiLyte 488
iFluor 488
KIRAVIA Blue 520
LysoBrite Green
LysoView 488
MaxLight 490
MemBrite Fix 488/515
MitoLite Green EX488
MitoLite Green FM
MitoMark Green
MitoROS 520
NBD
Neuro DiO
NL 493
NovaFluor Blue 530
Nuclear Green
Nuclear Orange
NucSpot 470
NucSpot Live 488
Oxazole Yellow
Oxazole Yellow Homodimer
Oyster 488
PhenoVue 493 Lipid Stain
PhenoVue 503 Lysosomal Stain
PhenoVue 512 Nucleic Acid Stain
PhenoVue Cal-520 AM Bright, Calcium Indicator
PhenoVue Cal-520 AM, Calcium Indicator
PhenoVue Fluo-4 AM, Calcium Indicator
PhenoVue Fluor 488
PhenoVue ROS-490, Total Oxidative Stress Indicator
PicoGreen
PromoFluor 488P
RealBlue 545
Spark Blue 550
Spark Blue 574
Spectrum Green
SureLight 488
Syto 16
Syto 24
Thiazole Orange Homodimer
Thiazole Orange Iodide
Vio 515
Vio Bright FITC
VRDye 490
Laser

561

--

595

560

Alexa Fluor 546
Alexa Fluor 555
Alexa Fluor 561
ATTO 542
ATTO 550
ATTO 565
ATTO Rho3B
BOBO-3
Calcein Red AM
Calcein Red-Orange AM
Calcium Orange
CellMask Orange Plasma Membrane Stain
CF568
CF570
cFluor BYG575
cFluor BYG610
cFluor YG584
cFluor YG610
CytoTrace Red
DyLight 549
DyLight 550
Dyomics 547
ExoBrite 560/585 EV Membrane Stain
HiLyte 555
LysoBrite Orange
LysoBrite Red
LysoView 550
MemBrite Fix 568/580
MitoLite Orange FX570
MitoLite Red FX600
MitoMark Red
mScarlet
NL 557
NovaFluor Yellow 570
PE
PE-Dazzle 594
PE-DyLight 594
PE-Dyomics 590
PE-eFluor 610
PhenoVue 551 Mitochondrial Stain
PhenoVue 577 Lysosomal Stain
PhenoVue 578 Mitochondrial Stain
PhenoVue Cal-590 AM Bright, Calcium Indicator
PhenoVue Cal-590 AM, Calcium Indicator
PhenoVue Fluor 555
PhenoVue ROS-570, Total Oxidative Stress Indicator
RealYellow 586
ReAsH
Rhodamine Red
Rhod-FF AM
SNARF 1 pH6.0
Spark YG 581
SureLight PE-594

--

640

595

ATTO 550
ATTO 565
ATTO Rho3B
BactoView Live Red
BOBO-3
Calcein Red-Orange AM
Calcium Orange
CellMask Orange Plasma Membrane Stain
CF568
CF570
cFluor BYG610
cFluor YG584
cFluor YG610
DyLight 549
Dyomics 547
ExoBrite 560/585 EV Membrane Stain
LipidSpot 610
LysoBrite Red
LysoView 550
LysoView 594
Magic Red
MemBrite Fix 568/580
MitoLite Orange FX570
MitoLite Red FX600
MitoMark Red
mNeptune
mScarlet
NovaFluor Yellow 610
NucSpot Far-Red
PE-Alexa 610
PE-ATTO 594
PE-CF594
PE-Dazzle 594
PE-DyLight 594
PE-Dyomics 590
PE-eFluor 610
PE-Fire 640
PE-Vio615
PhenoVue 551 Mitochondrial Stain
PhenoVue 577 Lysosomal Stain
PhenoVue 578 Mitochondrial Stain
PhenoVue Cal-590 AM Bright, Calcium Indicator
PhenoVue Cal-590 AM, Calcium Indicator
PhenoVue Nile Red Lipid Stain
RealYellow 586
ReAsH
Rhodamine Red
Rhod-FF AM
SNARF 1 pH6.0
SNARF 1 pH9.0
Spark YG 581
SureLight PE-594
SureLight RPC
Laser

642

--

745

640

Alexa Fluor 635
Alexa Fluor 647
Alexa Fluor 660
APC
APC-Alexa 680
APC-Alexa 700
APC-R700
ATTO 633
ATTO 647
ATTO 655
ATTO 665
Calcein Deep Red AM
CellMask Deep Red Plasma Membrane Stain
CellTracker Deep Red
CF633
CF640R
CF647
cFluor R685
Chromeo 642
CoraLite 647A
CoraLite Plus 647
Cy5
CypHer5E
DiIC1(5)
DRAQ 9
DyLight 633
DyLight 649
DyLight 650
Dyomics 647
ExoBrite 640/660 EV Membrane Stain
FluoroProbes647h
HiLyte 647
iFluor 633
iFluor 647
IRDye 650
IRDye 680LT
IRDye 680RD
Janelia Fluor 635
Janelia Fluor 646
Janelia Fluor 669
LipidSpot 610
LysoBrite NIR
LysoView 640
LysoView 650
LysoView 680
MaxLight 650
MemBrite Fix 640/660
MemBrite Fix 660/680
MemBrite Fix ST 650/665
MemBrite Fix ST 667/685
MitoLite Deep Red FX660
MitoLite NIR FX690
MitoView 650
MitoView Fix 640
NL 637
NucSpot 640
NucSpot 680
NucSpot Live 650
Oyster 650
PhenoVue 641 Mitochondrial Stain
PhenoVue Fluor 647
PhenoVue ROS-670, Total Oxidative Stress Indicator
PromoFluor 647P
Red 718
Seta-APC-705
Spark NIR 685
STAR 635
STAR 635P
STAR RED
SureLight APC
SureLight P3
Thiazole Red
Thiazole Red Homodimer
Vio 667
Vio Bright 667
Vio Bright R667
Vio Bright R720
Vio R667
Vio R720

--

800

745

APC-CF750
APC-CF770
APC-eFluor 780
APC-Fire 750
APC-Fire 810
APC-H7
APC-Vio770
DRAQ 9
LysoView 680
PE-Fire 780
Seta-APC-780

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.