University of Alberta
Faculty of Medicine and Dentistry (FoMD) Flow Cytometry Core

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Fortessa X-20 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

Brilliant Ultraviolet 395

740 / 35

--

Brilliant Ultraviolet 737
Laser

405

450 / 20

--

AbBy Fluor 405
ABflo 405
ABflo 450
Aco-490
Alexa Fluor 405
CF405M
cFluor V450
CoraLite Plus 405
CytoCalcein Violet 450
DY-415
DyLight 405
eFluor 450
ExoBrite 410/450 EV Membrane Stain
HiLyte 405
iFluor 405
LysoView 405
MaxLight 405
MemBrite Fix 405/430
mFluor Violet 450
MitoLite Blue FX490
MitoView 405
Nuclear Violet
Oxazole Blue Homodimer
Pacific Blue
POPO-1
PromoFluor 405
RayBright Violet 450
Spark Violet 423
Spectrum Aqua
StarBright Violet 440
V450
Vio Blue
Vio Bright V423
violetFluor 450

525 / 50

--

AmCyan
Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

610 / 20

--

Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

--

Brilliant Violet 650
Chlorophyll A
QM-FN-SO3
StarBright Violet 670
SuperBright 645
SuperBright 702
eFluor 650NC
eVolve 655
Qdot 655

710 / 50

--

Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

--

Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

--

FITC
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

695 / 40

--

ATTO 490LS
Brilliant Blue 700
cFluor B690
cFluor BYG667
cFluor BYG680
cFluor BYG710
Chromeo 494
Di-8-ANEPPS
NovaFluor Blue 660 / 40S
NovaFluor Blue 690
PE-Cy5
PE-Cy5.5
PE-Dyomics 647
PE-Fire 700
PerCP-eFluor 710
PerCP-Vio700
PE-Vio670
QM-FN-SO3
RealBlue 670
RealBlue 705
RMR-Tre
RPE-CF647T
StarBright Blue 675
StarBright Blue 700
SureLight PE Cyanine 5.0
SureLight PE Cyanine 5.5
Laser

561

586 / 15

--

PE
CF550R
CF568
CF570

610 / 20

--

PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

--

PE-Cy5

710 / 50

--

PE-Cy5.5

780 / 60

--

PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

AbBy Fluor 647
ABflo 647
Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
Alexa Fluor 660
APC
ATTO 633
ATTO 643
ATTO 647
ATTO 647N
ATTO 665
Calcein Deep Red AM
CellMask Deep Red Plasma Membrane Stain
CellTracker Deep Red
CellVue Claret
CF633
CF640R
CF647
cFluor R659
cFluor R668
cFluor R685
Chromeo 642
CoraLite 647
CoraLite 647A
CoraLite Plus 647
Cy5
CypHer5E
DiIC1(5)
DRAQ 9
DyLight 633
DyLight 649
DyLight 650
Dyomics 647
ExoBrite 640/660 EV Membrane Stain
FluoroProbes647h
HiLyte 647
iFluor 633
iFluor 647
IRDye 650
IRDye 680LT
IRDye 680RD
Janelia Fluor 630b, Maleimide
Janelia Fluor 630b, NHS ester
Janelia Fluor 635
Janelia Fluor 635b, Maleimide
Janelia Fluor 635b, NHS ester
Janelia Fluor 635, Tetrazine
Janelia Fluor 646
Janelia Fluor 669
LipidSpot 610
LysoBrite NIR
LysoTracker Deep Red
LysoView 640
LysoView 650
MaxLight 650
MemBrite Fix 640/660
MemBrite Fix 660/680
MemBrite Fix ST 650/665
MemBrite Fix ST 667/685
MitoLite Deep Red FX660
MitoLite NIR FX690
MitoView 650
MitoView Fix 640
NL 637
NucSpot 640
NucSpot Far-Red
NucSpot Live 650
Oyster 650
PhenoVue 641 Mitochondrial Stain
PhenoVue Fluor 647
PhenoVue ROS-670, Total Oxidative Stress Indicator
PromoFluor 647P
RayBright APC
RayBright Red 647
SiRA 2
Spark NIR 685
STAR 635
STAR 635P
STAR RED
SureLight APC
SureLight P3
SureLight RPC
Thalidomide-Cyanine 5
Thiazole Red
Thiazole Red Homodimer
Vio 667
Vio Bright 667
Vio Bright R667
Vio R667
XFD647

730 / 45

--

Alexa Fluor 700
APC-R700

780 / 60

--

APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.