University of Southern California (USC)
USC Flow Cytometry Core Facility

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Bio-Rad S3e 4 color 488/561 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

488

525 / 30

--

 Alexa Fluor 488
CFSE
DyLight 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

586 / 24

--

 PE

615 / 25

--

 PE-Texas Red
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

--

655

 PE-Alexa 647
Laser

561

586 / 24

--

 PE
CF550R
CF568
CF570

615 / 25

--

 DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-Texas Red

--

655

 PE-Alexa 647
Vybrant DyeCycle Ruby
7-AAD

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.