University of Southern California (USC)
USC Flow Cytometry Core Facility

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BD FACS Aria IIu Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

--

 Alexa Fluor 350
Alexa Fluor 405
AMCA
DAPI
DyLight 350
DyLight 405
Hoechst (33258)
Hoechst (33342)
Indo-Hi (Ca2+bound)
Vybrant DyeCycle Violet

530 / 30

--

505

 Brilliant Ultraviolet 496
Indo-Lo (Ca2+free)
Live/Dead Fix Aqua
Qdot 545
Qdot 705

--

675

630

 Brilliant Ultraviolet 737
Hoechst (33342)
Propidium Iodide
Qdot 705
Laser

488

510 / 20

--

505

 Alexa Fluor 488
Brilliant Blue 515
CFSE
Cy2
DiO
DyLight 488
FITC
Fluorescein
GFP
Live/Dead Fix Green
Vio Bright FITC

550 / 30

--

535

 Alexa Fluor 532
mBanana
Vybrant DyeCycle Green
Vybrant DyeCycle Orange
YFP

610 / 20

--

600

 Ethidium B.

695 / 40

--

635

 PerCP
Laser

561

582 / 15

--

--

 Alexa Fluor 568
CFP
Cy3.5
dsRed
dTomato
mTangerine
PE
RFP
CF550R
CF568

610 / 20

--

595

 DyLight 594
ECD
mCherry
PE-Alexa 610
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Texas Red
Texas Red
YOYO-3

670 / 14

--

635

 mKate2
PE-Alexa 647

710 / 50

--

685

 PE-Cy5.5

780 / 60

--

750

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

633

660 / 20

--

--

 Alexa Fluor 633
Alexa Fluor 647
APC
Cy5
DyLight 633
NL 637
Sytox Red
TOTO-3
Alexa Fluor 635
CF640R

730 / 45

--

685

 Alexa Fluor 660
Alexa Fluor 680
APC-Cy5.5
DyLight 680

780 / 60

--

755

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770
DRAQ 5

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.