University of Southern California (USC)
USC Flow Cytometry Core Facility

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BD FACSAria IIIu Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Pacific Blue
Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

530 / 30

502

 Alexa Fluor 430
Pacific Orange
Brilliant Violet 510
DyLight 405LS
Krome Orange
V500
Vio Green

616 / 23

600

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 14

655

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

730 / 45

690

 Qdot 705

780 / 60

755

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

510 / 20

502

 Alexa Fluor 488
CFSE
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
FITC
Fluorescein

695 / 40

535

 PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

582 / 15

--

 Cy3
dsRed
PE
Alexa Fluor 546
Alexa Fluor 555
CF550R
CF568
CF570
Cyanine 3 SE
TRITC

610 / 20

600

 PE-Alexa 610
Propidium Iodide
Texas Red

670 / 14

630

 PE-Cy5

710 / 50

685

 PE-Alexa 647
PE-Cy5.5

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 633
APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

730 / 45

690

 Alexa Fluor 660
Alexa Fluor 680
APC-Cy5.5
DyLight 680

780 / 60

755

 APC-Cy7
DRAQ 5
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.