CT26

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Institution: University of Pittsburgh
Facility: UPMC Hillman Cancer Center Cytometry Facility
Machine: Fortessa
  Antigen Channel Fluorophore Supplier Details
F4/80 α-Mouse 628nm
670/30 		APC
          		Bio-Rad
MCA497APC
Rat IgG2b
CI:A3-1
100 Tests
$404.00 		Supplier Page
FOXP3 α-Mouse 405nm
450/50 		Brilliant Violet 421
          		BioLegend
126419
Rat IgG2b κ
MF-14
50 μg
$253.00 		Supplier Page
CD274 (PD-L1) α-Mouse 561nm
582/15 		PE
          		BD Biosciences
558091
Rat IgG2a λ
MIH5
100 μg
$252.00 		Supplier Page
Ly-6G α-Mouse 561nm
610/20 		PE-CF594
          		BD Biosciences
562700
Rat IgG2a κ
1A8
50 μg
$232.00 		Supplier Page
TCR beta α-Mouse 561nm
780/40 		PE-Cy7
          		BioLegend
109222
Armenian Hamster IgG
H57-597
100 μg
$220.00 		Supplier Page
CD8a α-Mouse 488nm
515/30 		FITC
          		BioLegend
100706
Rat IgG2a κ
53-6.7
500 μg
$99.00 		Supplier Page
Ly-6C α-Mouse 405nm
710/45 		Brilliant Violet 711
          		BioLegend
128037
Rat IgG2c κ
HK1.4
50 μg
$286.00 		Supplier Page
CD11b α-Mouse 405nm
515/20 		Brilliant Violet 510
          		BioLegend
101245
Rat IgG2b κ
M1/70
125UL
$165.00 		Supplier Page
CD4 α-Mouse 488nm
710/50 		PerCP-Cy5.5
          		BioLegend
100434
Rat IgG2b κ
GK1.5
100 μg
$323.00 		Supplier Page
Complexity Index: 5.16

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Spillover Matrix
 
APC
Brilliant Violet 421
PE
PE-CF594
PE-Cy7
FITC
Brilliant Violet 711
Brilliant Violet 510
PerCP-Cy5.5
  670/30 450/50 582/15 610/20 780/40 515/30 710/45 515/20 710/50
F4/80 APC 670/30 48.2 % 0.0 % 0.0 % 0.7 % 0.0 % 0.0 % 0.0 % 0.0 % 0.3 %
FOXP3 Brilliant Violet 421 450/50 0.0 % 34.0 % 0.0 % 0.0 % 0.0 % 0.0 % 0.0 % 2.1 % 0.0 %
CD274 (PD-L1) PE 582/15 0.0 % 0.0 % 71.8 % 22.7 % 0.0 % 0.0 % 0.0 % 0.0 % 0.5 %
Ly-6G PE-CF594 610/20 0.2 % 0.0 % 16.7 % 86.5 % 0.0 % 0.0 % 0.1 % 0.0 % 2.9 %
TCR beta PE-Cy7 780/40 0.2 % 0.0 % 47.6 % 12.8 % 76.1 % 0.0 % 0.0 % 0.0 % 1.4 %
CD8a FITC 515/30 0.0 % 0.0 % 0.0 % 0.0 % 0.0 % 63.3 % 0.0 % 4.7 % 0.0 %
Ly-6C Brilliant Violet 711 710/45 1.5 % 0.0 % 0.0 % 0.0 % 0.3 % 0.0 % 77.9 % 0.0 % 2.4 %
CD11b Brilliant Violet 510 515/20 0.0 % 50.3 % 0.0 % 0.0 % 0.0 % 0.0 % 0.9 % 96.2 % 0.0 %
CD4 PerCP-Cy5.5 710/50 32.4 % 0.0 % 0.0 % 0.0 % 7.0 % 0.0 % 43.1 % 0.0 % 62.5 %
Filter coverage is shown on the diagonals.
Gradient shadings indicate higher levels of spillover.