Ecole Polytechnique Federale de Lausanne (EPFL)
EPFL Flow Cytometry Core Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/epfl-flow
Logo: FluoroFinder

LSRII SORP Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
DAPI
Hoechst (33342)
Live/Dead Fix Blue
Vybrant DyeCycle Violet
Zombie UV
CF350
DyLight 350

--

670

 Hoechst (33342)
Propidium Iodide
Laser

405

450 / 50

--

 Brilliant Violet 421
CellTrace Violet
eFluor 450
Pacific Blue
Vio Blue
Vybrant DyeCycle Violet
Alexa Fluor 405
Cascade Blue
CF405M
DyLight 405

525 / 50

505

 Brilliant Violet 510
Krome Orange
Live/Dead Fix Aqua
Pacific Green
Qdot 525
Vio Green
Zombie Aqua
Alexa Fluor 430
AmCyan
Cascade Yellow

610 / 20

595

 Brilliant Violet 605
eFluor 605NC
eVolve 605
Live/Dead Fix Yellow
Qdot 605
Zombie Yellow
DyLight 405LS
SuperBright 600

670 / 25

630

 Brilliant Violet 650
eVolve 655
Qdot 655
eFluor 650NC
SuperBright 645

710 / 50

670

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

755

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

525 / 50

505

 Alexa Fluor 488
Brilliant Blue 515
CFSE
DyLight 488
FITC
GFP
MitoSpy Green
MitoTracker Green
Vio Bright FITC
YFP

685 / 35

675

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
7-AAD
Brilliant Blue 700
PE-Cy5
PE-Cy5.5
Laser

561

585 / 15

--

 Alexa Fluor 555
dsRed
MitoSpy Orange
MitoTracker Orange
PE
RFP
tdTomato
TRITC
CF550R
CF568

610 / 20

600

 DyLight 594
mCherry
MitoTracker Red
PE-Alexa 610
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Texas Red
PE-Vio615
Propidium Iodide

660 / 20

635

 MitoTracker Deep Red
PE-Cy5
7-AAD

710 / 50

685

 PE-Cy5.5

780 / 60

755

 PE-Cy7
PE-Vio770
Laser

640

670 / 14

--

 Alexa Fluor 647
APC
DyLight 650
eFluor 660
Live/Dead Fix Far Red
MitoTracker Deep Red
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647

710 / 50

685

 Alexa Fluor 700
DRAQ 5
DRAQ 7
Vybrant DyeCycle Ruby
APC-R700

780 / 60

755

 APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-Vio770
Live/Dead Fix Near IR (775)
Zombie NIR
APC-Alexa 750
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.