Emory University
Emory + Pediatric's/Winship Flow Cytometry Core

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FACSymphony A3 - Liberator Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395
Indo-Hi (Ca2+bound)

515 / 30

--

 Brilliant Ultraviolet 496
Hoechst (33342)
Indo-Lo (Ca2+free)

586 / 15

--

 Brilliant Ultraviolet 563

670 / 30

--

 Brilliant Ultraviolet 661

740 / 35

--

 Brilliant Ultraviolet 737

820 / 60

--

 Brilliant Ultraviolet 805
Laser

405

431 / 28

--

 Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
Pacific Blue
SuperBright 436
V450
Vio Blue

470 / 15

--

 Brilliant Violet 480
Brilliant Violet 510
eFluor 450
Pacific Blue
V450
CF405M
CF430
CF440
Vio Blue

586 / 15

--

 Brilliant Violet 570
Live/Dead Fix Aqua
Pacific Orange
Alexa Fluor 430
DyLight 405LS
Krome Orange
Qdot 585

610 / 20

--

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

670 / 30

--

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

710 / 50

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

740 / 35

--

 Brilliant Violet 750

780 / 60

--

 Brilliant Violet 786
Qdot 800
Laser

488

515 / 20

--

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
GFP
YFP
ATTO 488
CF488A
CF503R
Cy2

610 / 20

--

 Brilliant Blue 630-P2

670 / 30

--

 Brilliant Blue 660-P2

710 / 50

--

 PerCP-Cy5.5
PerCP-eFluor 710
Propidium Iodide
7-AAD
Brilliant Blue 700
PerCP-Vio700

750 / 30

--

 Brilliant Blue 755-P

780 / 60

--

 Brilliant Blue 790-P
Laser

561

586 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

--

 Katushka2S
mCherry
mKate2
PE-CF594
PE-Texas Red
RFP
CF583
CF583R
DyLight 594
ECD

670 / 30

--

 PE-Cy5
7-AAD

710 / 50

--

 PE-Cy5.5
7-AAD

780 / 60

--

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 633
Alexa Fluor 647
APC
eFluor 660
TO-Pro 3
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633

710 / 50

--

 Alexa Fluor 700
APC-R700

780 / 60

--

 APC-Cy7
APC-eFluor 780
APC-H7
Zombie NIR
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.