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Cy5.5

Cy5.5 (Ex-Max 678 nm/Em-Max 695 nm)

Cy5.5 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 678 nm and an emission peak at 695 nm. Spectrally its similar to Alexa Fluor™ 680.

Excitation & emission spectra

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Key facts

Excitation maximum
678 nm
Emission maximum
695 nm
Optimal laser line
660
Optimal filter
695/40
Relative brightness
1 of 5
Molecular weight
1318
Commercial antibodies
6.8k from 12 suppliers

Cy5.5 has an excitation maximum at 678 nm and an emission maximum at 695 nm, a Stokes shift of 17 nm. It is optimally excited by the 660 laser line.

Frequently asked questions

What is Cy5.5?
Cy5.5 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 678 nm and an emission peak at 695 nm. Spectrally its similar to Alexa Fluor™ 680.
What laser excites Cy5.5?
Cy5.5 is optimally excited by the 660 laser line. Its excitation maximum is 678 nm.
What filter should I use to detect Cy5.5?
A 695/40 filter captures the peak of the Cy5.5 emission near 695 nm with minimal spillover.
How bright is Cy5.5?
Cy5.5 has a relative brightness of 1 out of 5.
How many antibodies are available conjugated to Cy5.5?
FluoroFinder tracks 6796 commercially available Cy5.5 conjugates, searchable by target, clone, host and application.