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FVS700

FVS700 (Ex-Max 655 nm/Em-Max 696 nm)

FVS700 (Fixable Viability Stain 700) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 655 nm and an emission peak at 696 nm. In flow cytometry, this probe is excited using the 633/640 nm red laser.

Excitation & emission spectra

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Key facts

Excitation maximum
655 nm
Emission maximum
696 nm
Optimal laser line
628-640
Optimal filter
695/40
Commercial antibodies
1 from 1 supplier

FVS700 has an excitation maximum at 655 nm and an emission maximum at 696 nm, a Stokes shift of 41 nm. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is FVS700?
FVS700 (Fixable Viability Stain 700) from BD Biosciences is an amine-reactive viability dye used in flow cytometry when it will be necessary to fix and/or permeabilize the cells after staining. This reagent has an excitation peak at 655 nm and an emission peak at 696 nm. In flow cytometry, this probe is excited using the 633/640 nm red laser.
What laser excites FVS700?
FVS700 is optimally excited by the 628-640 laser line. Its excitation maximum is 655 nm.
What filter should I use to detect FVS700?
A 695/40 filter captures the peak of the FVS700 emission near 696 nm with minimal spillover.
How many antibodies are available conjugated to FVS700?
FluoroFinder tracks 1 commercially available FVS700 conjugates, searchable by target, clone, host and application.