mRuby
mRuby (Ex-Max 558 nm/Em-Max 600 nm)
<html>mRuby is a monomeric red fluroescent protein derived from <i>Entacmaea quadricolor. </i>It has excitation/ emissionp peaks at 558/600 nm, respectively.</html>
Excitation & emission spectra
Key facts
- Excitation maximum
- 558 nm
- Emission maximum
- 600 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
mRuby has an excitation maximum at 558 nm and an emission maximum at 600 nm, a Stokes shift of 42 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is mRuby?
- <html>mRuby is a monomeric red fluroescent protein derived from <i>Entacmaea quadricolor. </i>It has excitation/ emissionp peaks at 558/600 nm, respectively.</html>
- What laser excites mRuby?
- mRuby is optimally excited by the 561 laser line. Its excitation maximum is 558 nm.
- What filter should I use to detect mRuby?
- A 610/20 filter captures the peak of the mRuby emission near 600 nm with minimal spillover.